Portable paper-based device for quantitative colorimetric assays relying on light reflectance principle

被引：65
作者：
Li, Bowei [1 ]
Fu, Longwen [1 ]
Zhang, Wei [1 ,2 ]
Feng, Weiwei [1 ]
Chen, Lingxin [1 ]
机构：
[1] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
来源：
ELECTROPHORESIS | 2014年 / 35卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Colorimetric assay; Light reflectance; Metal ions; Nitrite; Paper-based microfluidic device; Point-of-care; LARGE-SCALE INTEGRATION; HEAVY-METAL IONS; MICROFLUIDIC DEVICES; LOW-COST; ELECTROCHEMICAL DETECTION; DEVELOPING-WORLD; SENSOR ARRAY; IONIZATION; DIAGNOSIS; BIOASSAYS;
D O I：
10.1002/elps.201300583
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper presents a novel paper-based analytical device based on the colorimetric paper assays through its light reflectance. The device is portable, low cost (<20 dollars), and lightweight (only 176 g) that is available to assess the cost-effectiveness and appropriateness of the original health care or on-site detection information. Based on the light reflectance principle, the signal can be obtained directly, stably and user-friendly in our device. We demonstrated the utility and broad applicability of this technique with measurements of different biological and pollution target samples (BSA, glucose, Fe, and nitrite). Moreover, the real samples of Fe (II) and nitrite in the local tap water were successfully analyzed, and compared with the standard UV absorption method, the quantitative results showed good performance, reproducibility, and reliability. This device could provide quantitative information very conveniently and show great potential to broad fields of resource-limited analysis, medical diagnostics, and on-site environmental detection.
引用
收藏
页码：1152 / 1159
页数：8
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