Molecular profiling of rice (Oryza sativa L.) genotypes differing in micronutrients (iron and zinc) content

被引：2
作者：
Brar, B. [1 ]
Jain, S. [2 ]
Jain, R. K. [1 ]
机构：
[1] CCS Haryana Agr Univ, Dept Biotechnol & Mol Biol, Hisar 125004, Haryana, India
[2] CCS Haryana Agr Univ, Dept Biochem, Hisar 125004, Haryana, India
来源：
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING | 2014年 / 74卷 / 01期
关键词：
Genetic diversity; microsatellite marker; micronutrient; Oryza sativa L; LENGTH POLYMORPHISMS; GENETIC DIVERSITY; DNA POLYMORPHISM; SSR MARKERS; AFLP; VARIETIES; CLONING; BASMATI;
D O I：
10.5958/j.0975-6906.74.1.011
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The present study was undertaken to study the genetic diversity among fourteen rice genotypes, micronutrient rich indica, Basmati, japonica and commercial cultivars using 50 microsatellite markers uniformly distributed across the rice genome. A total of 257 alleles were detected that ranged from 1 to 9 with an average of 5.14 alleles per locus. The overall size of amplified PCR products ranged from 73 to 585 bp. The Polymorphic Information Content (PIC) value ranged from 0 to 0.862 with an average of 0.66. The NTSYS-pc UPGMA tree cluster as well as WINBOOT analysis placed the Fe-rich genotypes with Zn-rich Jaya and high-yielding indica rice genotypes, Fe-rich Taraori Basmati in Basmati rice group with Pusa Basmati 1 and Basmati 370, zinc rich TNG67 in japonica group. The profiting of the genotypes would pave the way for genetic improvement for micronutrients in rice.
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页码：81 / 85
页数：5
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