Response of Drosophila to Wasabi is mediated by painless, the fly homolog of mammalian TRPA1/ANKTM1

被引:137
作者
Al-Anzi, Bader
Tracey, W. Daniel, Jr.
Benzer, Seymour [1 ]
机构
[1] CALTECH, Div Biol 156 29, Pasadena, CA 91125 USA
[2] Duke Univ, Med Ctr, Dept Anesthesiol, Durham, NC 27710 USA
[3] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[4] Duke Univ, Med Ctr, Dept Neurobiol, Durham, NC 27710 USA
基金
美国国家科学基金会;
关键词
D O I
10.1016/j.cub.2006.04.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A number of repellent compounds produced by plants elicit a spicy or pungent sensation in mammals [1-16]. In several cases, this has been found to occur through activation of ion channels in the transient receptor potential (TRP) family [1-7]. We report that isothiocyanate (ITC), the pungent ingredient of wasabi, is a repellent to the insect Drosophila melanogaster, and that the painless gene, previously known to be required for larval nociception, is required for this avoidance behavior. A painless reporter gene is expressed in gustatory receptor neurons of the labial palpus, tarsus, and wing anterior margin, but not in olfactory receptor neurons, suggesting a gustatory role. Indeed, painless expression overlaps with a variety of gustatory-receptor gene reporters. Some, such as Gr66a, are known to be expressed in neurons that mediate gustatory repulsion [8-10]. painless mutants are not taste blind; they show normal aversive gustatory behavior with salt and quinine and attractive responses to sugars and capsaicin. The painless gene is an evolutionary homolog of the mammalian "wasabi receptor" TRPA1/ANKTM1 [6], also thought to be involved in nociception. Our results suggest that the stinging sensation of isothiocyanate is caused by activation of an evolutionarily conserved molecular pathway that is also used for nociception.
引用
收藏
页码:1034 / 1040
页数:7
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