TLR3, TLR4 and TLRs7-9 Induced Interferons Are Not Impaired in Airway and Blood Cells in Well Controlled Asthma

被引：37

作者：

Sykes, Annemarie ^{[1
,2
,3
,4
,5
]}

Edwards, Michael R. ^{[1
,2
,3
,4
]}

Macintyre, Jonathan ^{[1
,2
,3
,4
,5
]}

del Rosario, Ajerico ^{[1
,2
,3
,4
,5
]}

Gielen, Vera ^{[1
,2
,3
,4
]}

Haas, Jennifer ^{[1
,2
,3
,4
]}

Kon, Onn Min ^{[5
]}

McHale, Mark ^{[6
]}

Johnston, Sebastian L. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London, England

[2] MRC, London, England

[3] Asthma UK Ctr Allerg Mech Asthma, London, England

[4] Univ London Imperial Coll Sci Technol & Med, Ctr Resp Infect, London, England

[5] Imperial Coll Healthcare NHS Trust, London, England

[6] AstraZeneca R&D Charnwood, Resp & Inflammat Res Area, Loughborough, Leics, England

来源：

PLOS ONE | 2013年 / 8卷 / 06期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

INVASIVE PNEUMOCOCCAL DISEASE; BRONCHIAL EPITHELIAL-CELLS; MONONUCLEAR-CELLS; RHINOVIRUS; INFECTION; DEFICIENT; RESPONSES; CHILDREN; AGONISTS; ALPHA;

D O I：

10.1371/journal.pone.0065921

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Defective Rhinovirus induced interferon-beta and interferon-lambda production has been reported in bronchial epithelial cells from asthmatics but the mechanisms of defective interferon induction in asthma are unknown. Virus infection can induce interferon through Toll like Receptors (TLR) 3, TLR7 and TLR8. The role of these TLRs in interferon induction in asthma is unclear. This objective of this study was to measure the type I and III interferon response to TLR in bronchial epithelial cells and peripheral blood cells from atopic asthmatics and non-atopic non-asthmatics. Bronchial epithelial cells and peripheral blood mononuclear cells from atopic asthmatic and non-atopic non-asthmatic subjects were stimulated with agonists to TLR3, TLR4 & TLRs7-9 and type I and III interferon and pro-inflammatory cytokine, interleukin(IL)-6 and IL-8, responses assessed. mRNA expression was analysed by qPCR. Interferon proteins were analysed by ELISA. Pro-inflammatory cytokines were induced by each TLR ligand in both cell types. Ligands to TLR3 and TLR7/8, but not other TLRs, induced interferon-beta and interferon-lambda in bronchial epithelial cells. The ligand to TLR7/8, but not those to other TLRs, induced only type I interferons in peripheral blood mononuclear cells. No difference was observed in TLR induced interferon or pro-inflammatory cytokine production between asthmatic and non-asthmatic subjects from either cell type. TLR3 and TLR7/8,, stimulation induced interferon in bronchial epithelial cells and peripheral blood mononuclear cells. Interferon induction to TLR agonists was not observed to be different in asthmatics and non-asthmatics.

引用

页数：10

共 32 条

[1] Synthetic agonists of Toll-like receptors 7, 8 and 9 [J].