Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA

被引：101

作者：

Abe, Naoko ^{[1
]}

Hiroshima, Michio ^{[2
]}

Maruyama, Hideto ^{[1
,3
]}

Nakashima, Yuko ^{[1
]}

Nakano, Yukiko ^{[1
]}

Matsuda, Akira ^{[3
]}

Sako, Yasushi ^{[2
]}

Ito, Yoshihiro ^{[1
]}

Abe, Hiroshi ^{[1
,4
]}

机构：

[1] RIKEN Adv Sci Inst, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

[2] RIKEN Adv Sci Inst, Cellular Informat Lab, Wako, Saitama 3510198, Japan

[3] Hokkaido Univ, Fac Pharmaceut Sci, Sapporo, Hokkaido 060, Japan

[4] PRESTO Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 27期

关键词：

cyclization; mRNA; protein expression; RNA structures; synthetic biology; MESSENGER-RNA; PROTEIN-SYNTHESIS; RIBOSOME; SEQUENCE; INITIATION; BACTERIA; CODON;

D O I：

10.1002/anie.201302044

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Getting the runaround: Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7004 / 7008

页数：5

共 22 条

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