Rolling Circle Amplification in a Prokaryotic Translation System Using Small Circular RNA

被引：111

作者：

Abe, Naoko ^{[1
]}

Hiroshima, Michio ^{[2
]}

Maruyama, Hideto ^{[1
,3
]}

Nakashima, Yuko ^{[1
]}

Nakano, Yukiko ^{[1
]}

Matsuda, Akira ^{[3
]}

Sako, Yasushi ^{[2
]}

Ito, Yoshihiro ^{[1
]}

Abe, Hiroshi ^{[1
,4
]}

机构：

[1] RIKEN Adv Sci Inst, Nano Med Engn Lab, Wako, Saitama 3510198, Japan

[2] RIKEN Adv Sci Inst, Cellular Informat Lab, Wako, Saitama 3510198, Japan

[3] Hokkaido Univ, Fac Pharmaceut Sci, Sapporo, Hokkaido 060, Japan

[4] PRESTO Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 27期

关键词：

cyclization; mRNA; protein expression; RNA structures; synthetic biology; MESSENGER-RNA; PROTEIN-SYNTHESIS; RIBOSOME; SEQUENCE; INITIATION; BACTERIA; CODON;

D O I：

10.1002/anie.201302044

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Getting the runaround: Small circular RNA molecules containing an infinite open reading frame were synthesized and tested in an E. coli cell-free translation system. A circular RNA 126 nucleotides in length was found to produce more product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA in this continuous peptide synthesis. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7004 / 7008

页数：5

共 22 条

[1]

[Anonymous], 1999, ANGEW CHEM INT ED, V38, P3654

[2]

[Anonymous], 2008, ANGEW CHEM INT EDIT

[3]

[Anonymous], 2008, ANGEW CHEM INT ED, V47, P6330

[4] ROLLING-CIRCLE RNA-SYNTHESIS - CIRCULAR OLIGONUCLEOTIDES AS EFFICIENT SUBSTRATES FOR T7 RNA-POLYMERASE [J].

DAUBENDIEK, SL ;

RYAN, K ;

KOOL, ET .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (29) :7818-7819

[5]

Frieden M., 1999, ANGEW CHEM, V111, P3870

[6] An analysis of protein domain linkers: their classification and role in protein folding [J].

George, RA ;

Heringa, J .

PROTEIN ENGINEERING, 2002, 15 (11) :871-879

[7] Structural aspects of messenger RNA reading frame maintenance by the ribosome [J].

Jenner, Lasse B. ;

Demeshkina, Natalia ;

Yusupova, Gulnara ;

Yusupov, Marat .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2010, 17 (05) :555-U48

[8] Tandem repeats in proteins: From sequence to structure [J].

Kajava, Andrey V. .

JOURNAL OF STRUCTURAL BIOLOGY, 2012, 179 (03) :279-288

[9] Initiation of protein synthesis in bacteria [J].

Laursen, BS ;

Sorensen, HP ;

Mortensen, KK ;

Sperling-Petersen, HU .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2005, 69 (01) :101-+

[10]

Lee K, 1996, RNA, V2, P1270

← 1 2 3 →