A rapid assay for Hendra virus IgG antibody detection and its titre estimation using magnetic nanoparticles and phycoerythrin

被引:14
作者
Gao, Yuan [1 ]
Pallister, Jackie [2 ]
Lapierre, Florian [1 ]
Crameri, Gary [2 ]
Wang, Lin-Fa [2 ]
Zhu, Yonggang [1 ,3 ]
机构
[1] CSIRO Mfg Flagship, Clayton, Vic 3168, Australia
[2] CSIRO Biosecur Flagship, East Geelong, Vic 3219, Australia
[3] Melbourne Ctr Nanofabricat, Clayton, Vic 3169, Australia
关键词
Hendra virus; Antibody; Immunoassay; Magnetic nanoparticles; Phycoerythrin; NEUTRALIZING ANTIBODIES; G-GLYCOPROTEIN; NIPAH VIRUS; HENIPAVIRUS; DIAGNOSIS; EXPRESSION; PARTICLES;
D O I
10.1016/j.jviromet.2015.05.008
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Detection of Hendra viral IgG antibody in animal sera is useful for surveillance following a virus outbreak. The commonly used enzyme-linked immunosorbent assay and fluorescence-based Luminex assay typically consist of three steps and take at least several hours to complete. We have simplified the procedure to two steps in an effort to develop a rapid procedure for IgG antibody, but not IgM antibody, detection. This is achieved by conjugating the fluorescence label R-phycoerythrin directly onto the IgG binding protein Protein G. The use of magnetic nanoparticles, due to their large specific surface area, has helped reduce each of the binding steps to 20 min. As a result, the whole assay can be completed in 60 min. We also demonstrate a method to quickly estimate IgG antibody titres by assaying the sera at only two dilutions (i.e. 1:20 and 1:1000) and using the fluorescence ratio at these dilutions as an indicator of antibody titre. The results of this approach correlated well with the well-regarded serum neutralization test in virus antibody assays. This protocol reported here can be adopted in Luminex assays, fluorescence-linked immunosorbent assays and assays on microfluidics platforms for rapid antibody surveillance of Hendra and other viruses. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:170 / 177
页数:8
相关论文
共 37 条
[1]   Pseudoparticle neutralization is a reliable assay to measure immunity and cross-reactivity to H5N1 influenza viruses [J].
Alberini, Isabella ;
Del Tordello, Elena ;
Fasolo, Alba ;
Temperton, Nigel J. ;
Galli, Grazia ;
Gentile, Chiara ;
Montomoli, Emanuele ;
Hilbert, Anne K. ;
Banzhoff, Angelika ;
Del Giudice, Giuseppe ;
Donnelly, John J. ;
Rappuoli, Rino ;
Capecchi, Barbara .
VACCINE, 2009, 27 (43) :5998-6003
[2]   Bead Arrays for Antibody and Complement Profiling Reveal Joint Contribution of Antibody Isotypes to C3 Deposition [J].
Ayoglu, Burcu ;
Szarka, Eszter ;
Huber, Krisztina ;
Orosz, Anita ;
Babos, Fruzsina ;
Magyar, Anna ;
Hudecz, Ferenc ;
Rojkovich, Bernadette ;
Gati, Tamas ;
Nagy, Gyoergy ;
Schwenk, Jochen M. ;
Sarmay, Gabriella ;
Prechl, Jozsef ;
Nilsson, Peter ;
Papp, Krisztian .
PLOS ONE, 2014, 9 (05)
[3]   Neutralization assays for differential henipavirus serology using Bio-Plex Protein Array Systems [J].
Bossart, Katharine N. ;
McEachern, Jennifer A. ;
Hickey, Andrew C. ;
Choudhry, Vidita ;
Dimitrov, Dimiter S. ;
Eaton, Bryan T. ;
Wang, Lin-Fa .
JOURNAL OF VIROLOGICAL METHODS, 2007, 142 (1-2) :29-40
[4]   Receptor binding, fusion inhibition, and induction of cross-reactive neutralizing antibodies by a soluble G glycoprotein of Hendra virus [J].
Bossart, KN ;
Crameri, G ;
Dimitrov, AS ;
Mungall, BA ;
Feng, YR ;
Patch, JR ;
Choudhary, A ;
Wang, LF ;
Eaton, BT ;
Broder, CC .
JOURNAL OF VIROLOGY, 2005, 79 (11) :6690-6702
[5]   Functional expression and membrane fusion tropism of the envelope glycoproteins of Hendra virus [J].
Bossart, KN ;
Wang, LF ;
Eaton, BT ;
Broder, CC .
VIROLOGY, 2001, 290 (01) :121-135
[6]  
Charlermroj R., 2013, PLOS ONE, V8
[7]   Expression of truncated phosphoproteins of Nipah virus and Hendra virus in Escherichia coli for the differentiation of henipavirus infections [J].
Chen, Ji-Ming ;
Yaiw, Koon Chu ;
Yu, Meng ;
Wang, Lin-Fa ;
Wang, Qing-Hua ;
Crameri, Gary ;
Wang, Zhi-Liang .
BIOTECHNOLOGY LETTERS, 2007, 29 (06) :871-875
[8]   A comparative indirect ELISA for the detection of henipavirus antibodies based on a recombinant nucleocapsid protein expressed in Escherichia coli [J].
Chen, Ji-Ming ;
Yu, Meng ;
Morrissy, Chris ;
Zhao, Yong-Gang ;
Meehan, Greer ;
Sun, Ying-Xue ;
Wang, Qing-Hua ;
Zhang, Wei ;
Wang, Lin-Fa ;
Wang, Zhi-Liang .
JOURNAL OF VIROLOGICAL METHODS, 2006, 136 (1-2) :273-276
[9]   Characterization of Lassa Virus Cell Entry and Neutralization with Lassa Virus Pseudoparticles [J].
Cosset, Francois-Loic ;
Marianneau, Philippe ;
Verney, Geraldine ;
Gallais, Fabrice ;
Tordo, Noel ;
Pecheur, Eve-Isabelle ;
ter Meulen, Jan ;
Deubel, Vincent ;
Bartosch, Birke .
JOURNAL OF VIROLOGY, 2009, 83 (07) :3228-3237
[10]   Laboratory diagnosis of Nipah and Hendra virus infections [J].
Daniels, P ;
Ksiazek, T ;
Eaton, BT .
MICROBES AND INFECTION, 2001, 3 (04) :289-295