Identical MALDI TOF MS-derived peak profiles in a pair of isogenic SCCmec-harboring and SCCmec-lacking strains of Staphylococcus aureus

被引：30

作者：

Szabados, Florian ^{[1
]}

Kaase, Martin ^{[1
]}

Anders, Agnes ^{[1
]}

Gatermann, Soeren G. ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Dept Med Microbiol, Inst Hyg & Microbiol, D-44802 Bochum, Germany

来源：

JOURNAL OF INFECTION | 2012年 / 65卷 / 05期

关键词：

Staphylococcus aureus; MALDI-TOF MS; Isogenic; SCCmec; mecA; ASSISTED-LASER-DESORPTION/IONIZATION; RAPID IDENTIFICATION; MASS-SPECTROMETRY; TIME; MEC; DISCRIMINATION; RESISTANCE; DNA;

D O I：

10.1016/j.jinf.2012.06.010

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

MALDI-TOF MS-based peak differences in oxacillin-resistant Staphylococcus aureus and oxacillin-susceptible S. aureus isolates have been described previously. Unfortunately, these isolates were not isogenic with respect to their mecA gene. Ours is the first to use a SCCmec-harboring parent and a SCCmec-lacking daughter strain, with the same genetic background, to unequivocally rule out strain-specific protein peaks. We could not show differences in the peak profiles within the preset Biotyper settings used for MALDI-TOF-based identification in this pair of SCCmec-harboring parent and SCCmec-lacking daughter strains. (c) 2012 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

引用

页码：400 / 405

页数：6

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