Prestress and Area Compressibility of Actin Cortices Determine the Viscoelastic Response of Living Cells

被引:38
作者
Cordes, Andrea [1 ]
Witt, Hannes [2 ]
Gallemi-Perez, Aina [2 ]
Brueckner, Bastian [1 ]
Grimm, Florian [1 ,3 ]
Vache, Marian [1 ]
Oswald, Tabea [4 ]
Bodenschatz, Jonathan [1 ]
Flormann, Daniel [5 ]
Lautenschlaeger, Franziska [5 ,6 ]
Tarantola, Marco [2 ]
Janshoff, Andreas [1 ]
机构
[1] Georg August Univ Gottingen, Inst Phys Chem, D-37077 Gottingen, Germany
[2] MaX Planck Inst Dynam & Self Org, D-37077 Gottingen, Germany
[3] Abberior GmbH, D-37077 Gottingen, Germany
[4] Georg August Univ Gottingen, Inst Org & Biomol Chem, D-37077 Gottingen, Germany
[5] Leibniz Inst New Mat, D-66123 Saarbrucken, Germany
[6] Saarland Univ, NT Fac, Expt Phys, D-66123 Saarbrucken, Germany
关键词
CORTEX; MECHANICS; MEMBRANE; CYTOSKELETON; INDENTATION; MICROSCOPY; RHEOLOGY; MODULUS;
D O I
10.1103/PhysRevLett.125.068101
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
Shape, dynamics, and viscoelastic properties of eukaryotic cells are primarily governed by a thin, reversibly cross-linked actomyosin cortex located directly beneath the plasma membrane. We obtain time-dependent rheological responses of fibroblasts and MDCK II cells from deformation-relaxation curves using an atomic force microscope to access the dependence of cortex fluidity on prestress. We introduce a viscoelastic model that treats the cell as a composite shell and assumes that relaxation of the cortex follows a power law giving access to cortical prestress, area-compressibility modulus, and the power law exponent (fluidity). Cortex fluidity is modulated by interfering with myosin activity. We find that the power law exponent of the cell cortex decreases with increasing intrinsic prestress and area-compressibility modulus, in accordance with previous finding for isolated actin networks subject to external stress. Extrapolation to zero tension returns the theoretically predicted power law exponent for transiently cross-linked polymer networks. In contrast to the widely used Hertzian mechanics, our model provides viscoelastic parameters independent of indenter geometry and compression velocity.
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页数:5
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