Semi-automated direct elution of dried blood spots for the quantitative determination of guanfacine in human blood

被引：1
作者：
Li, Yuanyuan [1 ]
Henion, Jack [1 ]
Abbott, Richard
Wang, Phillip [2 ]
机构：
[1] Advion Bioanalyt Labs, Ithaca, NY USA
[2] Shire Dev LLC, Wayne, NJ 19087 USA
来源：
BIOANALYSIS | 2012年 / 4卷 / 12期
关键词：
MASS-SPECTROMETRY; BIOANALYTICAL METHOD; METHOD VALIDATION; SMALL MOLECULES; MS/MS; PHARMACOKINETICS; EXTRACTION; SAMPLES; DRUGS;
D O I：
10.4155/BIO.12.111
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: Direct analysis of dried blood spot (DBS) samples was investigated using a prototype semi-automated robotic device that allows the direct elution of sample spots from a DBS paper card to an online SPE cartridge. The eluted SPE samples were analyzed with high-performance LC-MS/MS. Results: A LLOQ of 0.01 ng/ml was achieved with a linear calibration range from 0.01 to 25 ng/ml. Optimal performance data were obtained from spotting the internal standard solution on the card before blood spotting. Internal standard addition from the system injector loop produced intra-assay inaccuracy of -9.0-7.3% and precision of 1.3-8.2%, and inter-assay inaccuracy of -3.5-3.9% and precision of 4.4-8.7%. Conclusion: Results demonstrated the feasibility of a semi-automated online rapid direct elution method that avoids manual extraction for DBS sample analysis using the online DBS-SPE system coupled to LC-MS/MS.
引用
收藏
页码：1445 / 1456
页数：12
相关论文
共 27 条
[1]  
Abu-Rabie P, 2011, BIOANALYSIS, V3, P2769, DOI [10.4155/BIO.11.270, 10.4155/bio.11.270]
[2]   Method of Applying Internal Standard to Dried Matrix Spot Samples for Use in Quantitative Bioanalysis [J].
Abu-Rabie, Paul ;
Denniff, Philip ;
Spooner, Neil ;
Brynjolffssen, Jan ;
Galluzzo, Paul ;
Sanders, Giles .
ANALYTICAL CHEMISTRY, 2011, 83 (22) :8779-8786
[3]   Direct Quantitative Bioanalysis of Drugs in Dried Blood Spot Samples Using a Thin-Layer Chromatography Mass Spectrometer Interface [J].
Abu-Rabie, Paul ;
Spooner, Neil .
ANALYTICAL CHEMISTRY, 2009, 81 (24) :10275-10284
[4]   Key elements of bioanalytical method validation for small molecules [J].
Bansal, Surendra ;
DeStefano, Anthony .
AAPS JOURNAL, 2007, 9 (01) :E109-E114
[5]   Application of dried blood spots combined with HPLC-MS/MS for the quantification of acetaminophen in toxicokinetic studies [J].
Barfield, Matt ;
Spooner, Neil ;
Lad, Rakesh ;
Parry, Simon ;
Fowles, Susan .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2008, 870 (01) :32-37
[6]   Use of Dried Plasma Spots in the Determination of Pharmacokinetics in Clinical Studies: Validation of a Quantitative Bioanalytical Method [J].
Barfield, Matthew ;
Wheller, Robert .
ANALYTICAL CHEMISTRY, 2011, 83 (01) :118-124
[7]   Pharmacokinetics of a Guanfacine extended-release formulation in children and adolescents with attention-deficit-hyperactivity disorder [J].
Boellner, Samuel W. ;
Permick, Michael ;
Fiske, Kimberly ;
Lyne, Andrew ;
Sbojaei, Amir .
PHARMACOTHERAPY, 2007, 27 (09) :1253-1262
[8]  
Crawford E, 2011, BIOANALYSIS, V3, P1217, DOI [10.4155/BIO.11.99, 10.4155/bio.11.99]
[9]  
Deglon J, 2000, J PHARM BIOMED ANAL, V49, P1034
[10]   Automated system for on-line desorption of dried blood spots applied to LC/MS/MS pharmacokinetic study of flurbiprofen and its metabolite [J].
Deglon, Julien ;
Thomas, Aurelien ;
Daali, Youssef ;
Lauer, Estelle ;
Samer, Caroline ;
Desmeules, Jules ;
Dayer, Pierre ;
Mangin, Patrice ;
Staub, Christian .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2011, 54 (02) :359-367
123