Functional role of Na+/H+ exchanger in Ca2+ influx mediated via human endothelin type A receptor stably expressed in Chinese hamster ovary cells

被引：6

作者：

Horinouchi, Takahiro ^{[1
]}

Miyake, Yumie ^{[1
]}

Nishiya, Tadashi ^{[1
]}

Nishimoto, Arata ^{[1
]}

Morishima, Shigeru ^{[2
]}

Muramatsu, Ikunobu ^{[2
]}

Miwa, Soichi ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Med, Dept Cellular Pharmacol, Sapporo, Hokkaido 0608638, Japan

[2] Univ Fukui, Div Pharmacol, Dept Biochem & Bioinformat Sci, Sch Med, Fukui 9101193, Japan

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2008年 / 107卷 / 04期

关键词：

endothelin type A receptor; Na+/H+ exchanger; intracellular free Ca2+ concentration;

D O I：

10.1254/jphs.08135SC

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

This study examines the functional role of Na+/H+ exchanger (NHE) in Ca2+ influx mediated by human endothelin type A receptor (ETAR) expressed in Chinese hamster ovary (CHO) cells. Endothelin-1 (ET-1) increased extracellular acidification rate (ECAR), which was abolished by 5-(N-ethyl-N-isopropyl)amiloride (EIPA), an NHE inhibitor. EIPA and KB-R7943, a Na+/Ca2+ exchanger (NCX) inhibitor, inhibited ET-1-induced Sustained increases in intracellular Ca2+ concentration ([Ca2+](i)), and EIPA had no effect on [Ca2+](i) after KB-R7943 treatment. ET-1 -elicited sustained [Ca2+](i) increase was Suppressed by reducing extracellular Na+ concentration. These results Suggest that possible coupling of NHE with NCX via Na+ transport is involved in ETAR-mediated Sustained [Ca2+](i) increase.

引用

页码：456 / 459

页数：4

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