Immune Responses Induced by Recombinant Bacillus Subtilis Expressing the Hemagglutinin Protein of H5N1 in chickens

被引：19
作者：
Mou, Chunxiao [1 ]
Zhu, Liqi [1 ]
Yang, Jingjing [1 ]
Xu, Wenwen [1 ]
Cheng, Xiaoying [1 ]
Yang, Qian [1 ]
机构：
[1] Nanjing Agr Univ, Coll Vet Med, Weigang 1, Nanjing 210095, Jiangsu, Peoples R China
来源：
SCIENTIFIC REPORTS | 2016年 / 6卷
基金：
中国国家自然科学基金;
关键词：
GROWTH-PERFORMANCE; INFLUENZA; VIRUS; DELIVERY; SYSTEM; VACCINATION; INFECTION; ADJUVANT; ANTIGENS; LIGANDS;
D O I：
10.1038/srep38403
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
To develop an effective, safe, and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI) H5N1, we have constructed a recombinant Bacillus subtilis strain (B. S.-HA) expressing the hemagglutinin (HA) protein. Then we evaluated the immune function in chicken bone marrow derived dendritic cells (BM-DCs), and the immune response after oral immunization. Our results show that the recombinant Bacillus subtilis B. S.-HA could be sampled by BM-DCs in vitro and increase the BM-DCs major histocompatibility complex (MHC) II phenotype. The weight, height of the small intestine villus, and lymphoid tissue area of the ileum increased significantly in B. S.-HA immunized chickens (P < 0.05 or P < 0.01). B. S.-HA induced the secretion of cytokines and the expression of Tolllike receptors in the trachea and small intestine (P < 0.05 or P < 0.01). In addition, B. S.-HA elevated the specific IgA titers in the trachea, IgG and HI antibody titers in serum (P < 0.05 or P < 0.01). Therefore, B. S.-HA provides a potential novel strategy and approach for developing an H5N1 vaccine.
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页数：11
相关论文
共 39 条
[1]   Bacillus subtilis A temperature resistant and needle free delivery system of immunogens [J].
Amuguni, Hellen ;
Tzipori, Saul .
HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2012, 8 (07) :979-986
[2]   Bacillus subtilis spores: A novel microparticle adjuvant which can instruct a balanced Th1 and Th2 immune response to specific antigen [J].
Barnes, Andrew G. C. ;
Cerovic, Vuk ;
Hobson, Philip S. ;
Klavinskis, Linda S. .
EUROPEAN JOURNAL OF IMMUNOLOGY, 2007, 37 (06) :1538-1547
[3]   Oral antigen exposure in extreme early life in lambs influences the magnitude of the immune response which can be generated in later life [J].
Buchanan, Rachelle M. ;
Mertins, Sonja ;
Wilson, Heather L. .
BMC VETERINARY RESEARCH, 2013, 9
[4]  
BURDIN N, 1995, J IMMUNOL, V154, P2533
[5]   Effects of Bacillus subtilis var. natto and Saccharomyces cerevisiae mixed fermented feed on the enhanced growth performance of broilers [J].
Chen, K. -L. ;
Kho, W. -L. ;
You, S. -H. ;
Yeh, R. -H. ;
Tang, S. -W. ;
Hsieh, C. -W. .
POULTRY SCIENCE, 2009, 88 (02) :309-315
[6]   Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein [J].
Chen, Z ;
Yoshikawa, T ;
Kadowaki, S ;
Hagiwara, Y ;
Matsuo, K ;
Asanuma, H ;
Aizawa, C ;
Kurata, T ;
Tamura, S .
JOURNAL OF GENERAL VIROLOGY, 1999, 80 :2559-2564
[7]  
Driks A, 1999, MICROBIOL MOL BIOL R, V63, P1
[8]   Bacillus subtilis as a tool for vaccine development:: from antigen factories to delivery vectors [J].
Ferreira, LCS ;
Ferreira, RCC ;
Schumann, W .
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2005, 77 (01) :113-124
[9]   Use of a promoter trap system in Bacillus anthracis and Bacillus subtilis for the development of recombinant protective antigen-based vaccines [J].
Gat, O ;
Inbar, I ;
Aloni-Grinstein, R ;
Zahavy, E ;
Kronman, C ;
Mendelson, I ;
Cohen, S ;
Velan, B ;
Shafferman, A .
INFECTION AND IMMUNITY, 2003, 71 (02) :801-813
[10]   H5N1 infection of the respiratory tract and beyond: a molecular pathology study [J].
Gu, Jiang ;
Xie, Zhigang ;
Gao, Zhancheng ;
Liu, Jinhua ;
Korteweg, Christine ;
Ye, Juxiang ;
Lau, Lok Ting ;
Lu, Jie ;
Gao, Zifen ;
Zhang, Bo ;
McNutt, Michael A. ;
Lu, Min ;
Anderson, Virginia M. ;
Gong, Encong ;
Yu, Albert Cheung Hoi ;
Lipkin, W. Ian .
LANCET, 2007, 370 (9593) :1137-1145
1234