Determination of zearalenone and ochratoxin a mycotoxins in grain by fluorescence polarization immunoassay

被引：28

作者：

Bondarenko, A. P. ^{[1
]}

Eremin, S. A. ^{[1
]}

机构：

[1] Lomonosov Moscow State Univ, Dept Chem, Moscow 119991, Russia

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2012年 / 67卷 / 09期

关键词：

mycotoxins; zearalenone; ochratoxin A; FPIA;

D O I：

10.1134/S1061934812090031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Rapid procedures are reported for the determination of zearalenone and ochratoxin A, which make possible the analysis of up to 10 grain samples within 20 min by fluorescence polarization immunoassay. The influence of various fluorescein-based tracers on the sensitivity of analysis is examined. The most promising was admitted the use of ethylenediamine thiocarbamoylfluorescein and aminomethylfluorescein. The detection limits of the method for zearalenone and ochratoxin A were 3 and 1.5 ng/mL for a model system and 15 and 10 mu g/kg for grain, respectively. The performed analysis of spiked grain samples demonstrated the recovery of mycotoxins at a level of 84-97%.

引用

页码：790 / 794

页数：5

共 16 条

[1] Preparation of conjugated antigens based on zearalenone carboxymethyloxime and their use in enzyme immunoassay [J].

Burkin, AA ;

Kononenko, GP ;

Soboleva, NA ;

Zotova, EV .

APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2000, 36 (03) :282-288

[2]

CHU FS, 1977, J ASSOC OFF ANA CHEM, V60, P791

[3] A fluorescence polarization immunoassay for the detection of zearalenone in corn [J].

Chun, Hyang Sook ;

Choi, Eun Hye ;

Chang, Hyun-Joo ;

Choi, Sung-Wook ;

Eremin, Sergey A. .

ANALYTICA CHIMICA ACTA, 2009, 639 (1-2) :83-89

[4]

Collins W.P., 1988, COMPLEMENTARY IMMUNO

[5] FLUORESCENCE POLARIZATION IMMUNOASSAY - THEORY AND EXPERIMENTAL METHOD [J].

DANDLIKER, WB ;

KELLY, RJ ;

DANDLIKER, J ;

FARQUHAR, J ;

LEVIN, J .

IMMUNOCHEMISTRY, 1973, 10 (04) :219-227

[6]

Eccleston JF, 2005, PROGR MED CHEM, V43, P19, DOI 10.1016/S0079-6468(05)43002-7

[7] Fluorescence Polarization/Anisotropy in Diagnostics and Imaging [J].

Jameson, David M. ;

Ross, Justin A. .

CHEMICAL REVIEWS, 2010, 110 (05) :2685-2708

[8]

Khabriev R.U., 2010, TOKSIKOLOGICHESKAYA

[9]

Lacowicz J.R., 2006, Principles of Fluorescence Spectroscopy

[10] Development of a monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip [J].

Liu, Biing-Hui ;

Tsao, Zih-Jay ;

Wang, Jing-Jhih ;

Yu, Feng-Yih .

ANALYTICAL CHEMISTRY, 2008, 80 (18) :7029-7035

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