The effects of caffeine on wound healing

被引:34
作者
Ojeh, Nkemcho [1 ]
Stojadinovic, Olivera [2 ]
Pastar, Irena [2 ]
Sawaya, Andrew [2 ]
Yin, Natalie [2 ]
Tomic-Canic, Marjana [2 ]
机构
[1] Univ West Indies, Fac Med Sci, Cave Hill Campus,POB 64, BB-11000 Bridgetown, St Michael, Barbados
[2] Univ Miami, Miller Sch Med, Dept Dermatol & Cutaneous Surg, Wound Healing & Regenerat Med Res Program, Miami, FL 33136 USA
关键词
Caffeine; Ex vivo model; Migration; Proliferation; Re-epithelialisation; Wound healing; ADENOSINE RECEPTORS; HUMAN KERATINOCYTES; CELL-PROLIFERATION; EXPRESSION; MODEL; FIBROBLASTS; INHIBITION; ACTIVATION; APOPTOSIS; FIBROSIS;
D O I
10.1111/iwj.12327
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
The purine alkaloid caffeine is a major component of many beverages such as coffee and tea. Caffeine and its metabolites theobromine and xanthine have been shown to have antioxidant properties. Caffeine can also act as adenosine-receptor antagonist. Although it has been shown that adenosine and antioxidants promote wound healing, the effect of caffeine onwound healing is currently unknown. To investigate the effects of caffeine on processes involved in epithelialisation, we used primary human keratinocytes, HaCaT cell line and ex vivo model of human skin. First, we tested the effects of caffeine on cell proliferation, differentiation, adhesion andmigration, processes essential for normal wound epithelialisation and closure. We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) proliferation assay to test the effects of seven different caffeine doses ranging from 0.1 to 5 mM. We found that caffeine restricted cell proliferation of keratinocytes in a dose-dependent manner. Furthermore, scratch wound assays performed on keratinocyte monolayers indicated dose-dependent delays in cell migration. Interestingly, adhesion and differentiation remained unaffected in monolayer cultures treated with various doses of caffeine. Using a human ex vivo wound healing model, we tested topical application of caffeine and found that it impedes epithelialisation, confirming in vitro data. We conclude that caffeine, which is known to have antioxidant properties, impedes keratinocyte proliferation and migration, suggesting that it may have an inhibitory effect on wound healing and epithelialisation. Therefore, our findings are more in support of a role for caffeine as adenosine-receptor antagonist that would negate the effect of adenosine in promoting wound healing.
引用
收藏
页码:605 / 613
页数:9
相关论文
共 33 条
  • [1] Azam Sonish, 2003, Med Sci Monit, V9, pBR325
  • [2] Caffeine Intake May Modulate Inflammation Markers in Trained Rats
    Barcelos, Romulo Pillon
    Souza, Mauren Assis
    Amaral, Guilherme Pires
    Stefanello, Silvio Terra
    Bresciani, Guilherme
    Fighera, Michele Rechia
    Antunes Soares, Felix Alexandre
    Barbosa, Nilda de Vargas
    [J]. NUTRIENTS, 2014, 6 (04): : 1678 - 1690
  • [3] The enigmatic effects of caffeine in cell cycle and cancer
    Bode, Ann M.
    Dong, Zigang
    [J]. CANCER LETTERS, 2007, 247 (01) : 26 - 39
  • [4] Protective effects of tea polyphenols and caffeine
    Camouse, Melissa M.
    Hanneman, Kaija K.
    Conrad, Edward P.
    Baron, Elmo D.
    [J]. EXPERT REVIEW OF ANTICANCER THERAPY, 2005, 5 (06) : 1061 - 1068
  • [5] Adenosine A2A receptors in diffuse dermal fibrosis pathogenic role in human dermal fibroblasts and in a murine model of scleroderma
    Chan, E. S. L.
    Fernandez, P.
    Merchant, A. A.
    Montesinos, M. C.
    Trzaska, S.
    Desai, A.
    Tung, C. F.
    Khoa, D. N.
    Pillinger, M. H.
    Reiss, A. B.
    Tomic-Canic, M.
    Chen, J. F.
    Schwarzschild, M. A.
    Cronstein, B. N.
    [J]. ARTHRITIS AND RHEUMATISM, 2006, 54 (08): : 2632 - 2642
  • [6] Cronstein Bruce N, 2011, F1000 Biol Rep, V3, P21, DOI 10.3410/B3-21
  • [7] Diaz-Munoz M., 2010, Central Nervous System Agents in Medicinal Chemistry, V10, P259
  • [8] The effects of energy beverages on cultured cells
    Doyle, Wayne
    Shide, Eric
    Thapa, Slesha
    Chandrasekaran, Vidya
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 2012, 50 (10) : 3759 - 3768
  • [9] Differential expression of adenosine receptors in human endothelial cells -: Role of A2B receptors in angiogenic factor regulation
    Feoktistov, I
    Goldstein, AE
    Ryzhov, S
    Zeng, DW
    Belardinelli, L
    Voyno-Yasenetskaya, T
    Biaggioni, I
    [J]. CIRCULATION RESEARCH, 2002, 90 (05) : 531 - 538
  • [10] Fredholm BB, 2001, PHARMACOL REV, V53, P527