Structure/function studies on enzymes in the diaminopimelate pathway of bacterial cell wall biosynthesis

被引：82

作者：

Born, TL ^{[1
]}

Blanchard, JS ^{[1
]}

机构：

[1] Yeshiva Univ Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 1999年 / 3卷 / 05期

关键词：

D O I：

10.1016/S1367-5931(99)00016-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Within the past 18 months work has continued on the structure and mechanisms of enzymes involved in the diaminopimelic acid/lysine biosynthetic pathway. A novel structure has been determined for a PLP-independent epimerase, and structures with bound substrates have been solved for two other enzymes. Additionally, new studies have appeared describing the chemical mechanisms of three enzymes in the pathway.

引用

页码：607 / 613

页数：7

共 21 条

[1] THE PROSITE DICTIONARY OF SITES AND PATTERNS IN PROTEINS, ITS CURRENT STATUS [J].

BAIROCH, A .

NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :3097-3103

[2] The conformational change and active site structure of tetrahydrodipicolinate N-succinyltransferase [J].

Beaman, TW ;

Blanchard, JS ;

Roderick, SL .

BIOCHEMISTRY, 1998, 37 (29) :10363-10369

[3]

BERGES DA, 1986, J BIOL CHEM, V261, P6160

[4] Hydrolysis of N-succinyl-L,L-diaminopimelic acid by the Haemophilus influenzae dapE-encoded desuccinylase:: Metal activation, solvent isotope effects, and kinetic mechanism [J].

Born, TL ;

Zheng, RJ ;

Blanchard, JS .

BIOCHEMISTRY, 1998, 37 (29) :10478-10487

[5] PURIFICATION AND MECHANISM OF ACTION OF PROLINE RACEMASE [J].

CARDINALE, GJ ;

ABELES, RH .

BIOCHEMISTRY, 1968, 7 (11) :3970-+

[6] Structural symmetry:: The three-dimensional structure of Haemophilus influenzae diaminopimelate epimerase [J].

Cirilli, M ;

Zheng, RJ ;

Scapin, G ;

Blanchard, JS .

BIOCHEMISTRY, 1998, 37 (47) :16452-16458

[7] Synthesis and evaluation of novel substrates and inhibitors of N-Succinyl-LL-diaminopimelate aminotransferase (DAP-AT) from Escherichia coli [J].

Cox, RJ ;

Sherwin, WA ;

Lam, LKP ;

Vederas, JC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (32) :7449-7460

[8]

GILVARG C, 1960, FED PROC, V19, P948

[9] EXPRESSION OF RECOMBINANT DIAMINOPIMELATE EPIMERASE IN ESCHERICHIA-COLI - ISOLATION AND INHIBITION WITH AN IRREVERSIBLE INHIBITOR [J].

HIGGINS, W ;

TARDIF, C ;

RICHAUD, C ;

KRIVANEK, MA ;

CARDIN, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 186 (1-2) :137-143

[10]

Hwang KY, 1999, NAT STRUCT BIOL, V6, P422

← 1 2 3 →