Headspace solid phase microextraction/gas chromatography-mass spectrometry combined to chemometric analysis for volatile organic compounds determination in canine hair: A new tool to detect dog contamination by visceral leishmaniasis

被引：25
作者：
de Oliveira, Lidia S. [1 ]
Rodrigues, Frederico de M. [1 ,2 ]
de Oliveira, Fabio S. [4 ]
Mesquita, Paulo R. R. [1 ]
Leal, Danielle C. [3 ]
Alcantara, Adriano C. [3 ]
Souza, Barbara M. [3 ]
Franke, Carlos R. [3 ]
Pereira, Pedro. A. de P. [1 ]
de Andrade, Jailson B. [1 ]
机构：
[1] Univ Fed Bahia, Inst Quim, BR-40170290 Salvador, BA, Brazil
[2] EBDA, Salvador, BA, Brazil
[3] Univ Fed Bahia, Escola Vet, BR-40170290 Salvador, BA, Brazil
[4] Univ Fed Reconcavo Bahia, Ctr Ciencias Saude, Santo Antonio De Jesus, BA, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 02期
关键词：
Canine hair; VOCs; HS-SPME/GC-MS; Multivariate optimization; PCA; Diagnosis; Visceral leishmaniasis;
D O I：
10.1016/j.jchromb.2008.09.028
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new analytical methodology using HS-SPME/GC-MS was optimized in order to attain maximum sensitivity, using multivariate Strategies. The proposed method was employed to evaluate the VOC profile exhaled from canine hair samples collected from 8 healthy dogs and from 16 dogs infected by Leishmania infantum. 274 VOCs were detected, which could be identified as aldehydes, ketones and hydrocarbons. After application of the Soft Independent Modeling of Class Analogy (SIMCA) and Principal Component Analysis (PCA) healthy and infected dogs, with similar VOCs profiles. Could be separately grouped, based On Compounds such as 2-hexanone, benzaldehyde, and 2.4-nonadienal. The proposed method is noninvasive, painless, readily accepted by dog owners and could be useful to identify several biomarkers with applications in the diagnosis of diseases. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：392 / 398
页数：7
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