A strip-based immunoassay for rapid determination of fenpropathrin

被引:25
作者
Chen, Xiujin [1 ,2 ]
Liu, Liqiang [1 ]
Kuang, Hua [1 ]
Song, Shanshan [1 ]
Xu, Chuanlai [1 ]
机构
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Henan Univ Sci & Technol, Food & Biol Engn Inst, Luoyang 471023, Peoples R China
关键词
LINKED-IMMUNOSORBENT-ASSAY; IMMUNOCHROMATOGRAPHIC ASSAY; PYRETHROID INSECTICIDES; CHROMATOGRAPHY; DELTAMETHRIN; CYHALOTHRIN; RESIDUES; MELAMINE;
D O I
10.1039/c3ay41030g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We report the generation of monoclonal antibodies against fenpropathrin, originating from a BALB/ c mouse immunized with a conjugate of hapten 1 [(RS)-cyano-3-phenoxybenzyl-2,2,3,3-tetramethylcyclopropane- carboxylic acid)] and keyhole limpet hemocyanin and the establishment of a monoclonal antibody-based immunochromatographic test strip. A coating antigen (test band) and a goat anti-mouse antibody (control band) were separately immobilized on nitrocellulose membrane strips as capture reagents. The anti-fenpropathrin monoclonal antibody was labeled with gold nanoparticles as a detection probe. This test strip had a wide linear range (15.6-250 mu g L-1) with a low detection limit of 62 +/- 6 mg L-1 for fenpropathrin, evaluated using a strip reader, and could be read within 10 min. This novel test strip was used to test fenpropathrin in fortified samples and found to have a high recovery rate (> 80%). In summary, our monoclonal antibody-based immunochromatographic strip offers a rapid screening tool for fenpropathrin detection in agricultural commodities.
引用
收藏
页码:6234 / 6239
页数:6
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