LONG-TERM STABLE RECORDING OF SINGLE-UNIT ACTIVITY IN THE AMYGDALA OF AWAKE RABBITS

The development of modern research technologies has made it possible to simultaneously monitor the activity of a large number of neurons involved in the work of distributed neural networks in the whole brain. An important aspect of such studies is the stability of recordings, which implies the possibility to record signal from the same cell for a long time. The aim of our work is to evaluate the usability of chronically implanted multiple microwires for stable recording of activity from single neurons in the rabbit brain. The studies were conducted on two adult European rabbits (Orictolagus cuniculus), which were implanted with bundles of microwires into the amygdala. Collection of experimental data was possible for 72 days in rabbit No 1; and for 964 days in rabbit No 2. The evaluation of the quality of registration of a neural signal showed that our design for the recording device provides for a longitudinal recording of the activity of single neurons with good quality. An automated approach made it possible to detect stably recorded single neurons, that in the future will allow a comprehensive study of the relationship between changes in brain cell activity and complex behavior.