Analyzing the repressive function of ultraspiracle, the Drosophila RXR, in Drosophila eye development

被引:16
作者
Ghbeish, N
McKeown, M
机构
[1] Salk Inst Biol Studies, Mol Biol & Virol Lab, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA
[3] Brown Univ, Dept Mol Biol Cell Biol & Biochem, Providence, RI 02912 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
Ultraspiracle; Ecdysone; Ecdysone Receptor; broad-complex; nuclear receptor; activation; repression; eye development; morphogenetic furrow;
D O I
10.1016/S0925-4773(01)00610-4
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Response to the insect hormone ecdysone is mediated by a nuclear receptor complex containing, Ultraspiracle (USP) and the Ecdysone Receptor (EcR). Among other phenotypes, loss of functional USP in Drosophila eye development results in an accelerated morphogenetic furrow, although loss of ecdysone arrests the furrow. We have shown that USP both represses and activates a gene affecting furrow movement, the ecdysone-responsive Z1 isoform. of Broad-Complex, and we report additional usp mutant phenotypes. Using targeted replacement of USP to rescue usp mutant clones in the eye, we have mapped various USP functions and tested whether the USP nuclear receptor has an activating as well as a repressive effect on furrow movement. Furrow movement and related phenotypes are rescued by the presence of USP in a limited domain near the furrow while other phenotypes are rescued by USP expression posterior to the furrow. Our data indicate roles for USP activity at multiple developmental stages and help explain why loss of functional USP leads to furrow advancement while loss of ecdysonc stops furrow movement. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
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页码:89 / 98
页数:10
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