Radio-modulatory effects of Green Tea Catechin EGCG on pBR322 plasmid DNA and murine splenocytes against gamma-radiation induced damage

被引：54

作者：

Richi, Benila ^{[1
]}

Kale, Raosaheb K. ^{[2
]}

Tiku, Ashu B. ^{[1
]}

机构：

[1] Jawaharlal Nehru Univ, Sch Life Sci, Radiat & Canc Therapeut Lab, New Delhi 110067, India

[2] Cent Univ Gujarat, Gandhinagar 382030, Gujarat, India

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2012年 / 747卷 / 01期

关键词：

Gamma-radiation; Polyphenols; Radioprotection; DNA damage; Epigallocatechin-3-gallate; Antioxidant; RAY-INDUCED SCISSION; FACTOR-KAPPA-B; IN-VITRO; EPIGALLOCATECHIN-GALLATE; IONIZING-RADIATION; POLYPHENOL (-)-EPIGALLOCATECHIN-3-GALLATE; OXIDATIVE DAMAGE; STRAND BREAKS; CELLS; ANTIOXIDANT;

D O I：

10.1016/j.mrgentox.2012.04.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Green tea is rich in polyphenols, like catechins, which are thought to contribute to the health benefits of tea. The aim of this study was to evaluate the radioprotective effect of EGCG (epigallocatechin-3-gallate), a green tea catechin on gamma-radiation induced cell damage. Under acellular condition of radiation exposure, pBR322 plasmid DNA was protected by EGCG in a concentration dependent manner. Treatment of murine splenocytes with EGCG 2 h prior to radiation (3 Gy), protected the cellular DNA against radiation-induced strand breaks. EGCG also inhibited gamma-radiation induced cell death in splenocytes. EGCG pretreatment to the cells decreased the radiation induced lipid peroxidation and membrane damage. The levels of phase II enzymes. glutathione and lactate dehydrogenase were restored with EGCG treatment prior to radiation. Our results show that pretreatment with EGCG offers protection to pBR322 DNA under acellular condition and normal splenocytes under cellular condition, against gamma-radiation induced damage and is better radioprotector in comparison to quercetin and vitamin C. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：62 / 70

页数：9

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