ISSR variations in eggplant (Solanum melongena L.) and related Solanum species

被引:50
|
作者
Isshiki, Shiro [1 ]
Iwata, Naoko [1 ]
Khan, Md. Mizanur Rahim [1 ]
机构
[1] Saga Univ, Fac Agr, Dept Appl Biol Sci, Saga 8408502, Japan
基金
欧盟地平线“2020”;
关键词
ISSR; eggplant; Solanum species; phylogenetic relationships; cultivar identification;
D O I
10.1016/j.scienta.2008.04.003
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Inter-simple sequence repeat (ISSR) analysis was performed in eight cultivars of eggplant (Solanum melongena) and 12 accessions in eight related Solanum species to evaluate the applicability of this analysis for assessing the phylogenetic relationships and identifying cultivars. A total of 552 polymorphic amplified bands were obtained from 34 of the 100 primers tested, and the percentage of polymorphisms was 99.1%. Cluster analysis based on the ISSR markers classified the Solanum species into seven groups: (i) S. melongena; (ii) S. aethiopicum and S. anguivi; (iii) S. incanum; (iv) S. violaceum and S. kurzii; (v) S. macrocarpon; (vi) S. virginianum and (vii) S. torvum. Combining the ISSR markers obtained by a few of the 34 primers was enough for distinguishing of the eight cultivars of eggplant. This ISSR analysis was demonstrated to be available for the phylogenetic study and the cultivar identification. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:186 / 190
页数:5
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