Expression of mucin 1 possessing a 3-sulfated core1 in recurrent and metastatic breast cancer

被引：32

作者：

Ideo, Hiroko ^{[1
,2
]}

Hinoda, Yuji ^{[3
]}

Sakai, Kohei ^{[3
]}

Hoshi, Ikue ^{[1
]}

Yamamoto, Shigeru ^{[4
]}

Oka, Masaaki ^{[4
]}

Maeda, Kazunari ^{[4
]}

Maeda, Noriko ^{[4
]}

Hazama, Shoichi ^{[4
]}

Amano, Junko ^{[2
]}

Yamashita, Katsuko ^{[1
]}

机构：

[1] Tokyo Inst Technol, Innovat Res Initiat, Yokohama, Kanagawa 227, Japan

[2] Noguchi Inst, Lab Glycobiol, Tokyo 1730003, Japan

[3] Yamaguchi Univ, Grad Sch Med, Dept Oncol & Lab Med, Yamaguchi, Japan

[4] Yamaguchi Univ, Grad Sch Med, Dept Digest Surg & Surg Oncol, Yamaguchi, Japan

来源：

INTERNATIONAL JOURNAL OF CANCER | 2015年 / 137卷 / 07期

基金：

日本学术振兴会;

关键词：

MUC1; galectin-4; 3-sulfated core1; breast cancer; tumor marker; metastasis; MONOCLONAL-ANTIBODIES; GALECTIN-4; LECTIN; RECOGNIZES; ANTIGENS; GLYCANS; BINDING;

D O I：

10.1002/ijc.29520

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Breast cancer is the most frequent cancer threatening the lives of women between the ages of 30 and 64. The cancer antigen 15-3 assay (CA15-3) has been widely used for the detection of breast cancer recurrence; however, its sensitivity and specificity are inadequate. We previously found that the breast cancer cell line YMBS secretes mucin 1 possessing 3-sulfated core1 (3Score1-MUC1) into the medium. Therefore, we here evaluated whether 3Score1-MUC1 is secreted into the blood streams of breast cancer patients, and whether it can serve as an improved breast cancer marker. We developed a lectin-sandwich immunoassay, called Gal4/MUC1, using a 3-sulfated core1-specific galectin-4 and a MUC1 monoclonal antibody. Using the Gal4/MUC1 assay method, we found that 3Score1-MUC1 was profoundly expressed in the blood streams of patients with recurrent and/or metastatic breast cancer. The positive ratio of the Gal4/MUC1 assay was higher than that of the CA15-3 assay in both primary (n=240) and relapsed (n=43) patients, especially in the latter of which the positive ratio of Gal4/MUC1 was 86%. whereas that of CA15-3 was 47%. Furthermore, serum Gal4/MUC1 levels could more sensitively reflect the recurrence of primary breast cancer patients after surgery. Therefore, the Gal4/MUC1 assay should be an excellent alternative to the CA15-3 tumor marker for tracking the recurrence and metastasis of breast cancer. What's new? The cancer antigen 15-3 (CA15-3) assay has been widely used for the detection of breast cancer recurrence; however, its sensitivity and specificity are inadequate. Here, the authors developed a lectin-sandwich immunoassay (Gal4/MUC1) using a 3-sulfated core1-specific galectin-4 and a MUC1 monoclonal antibody. They found that MUC1 possessing a 3-sulfated core1 (3Score1-MUC1) was expressed in the blood streams of patients with recurrent and/or metastatic breast cancer. The positive ratio of the Gal4/MUC1 assay was higher than that of the CA15-3 assay, especially in the relapsed or metastatic breast cancer. The study thus suggests the Gal4/MUC1 assay as an excellent alternative to CA15-3.

引用

页码：1652 / 1660

页数：9