OIP5-AS1/miR-137/ZNF217 Axis Promotes Malignant Behaviors in Epithelial Ovarian Cancer

被引：32
作者：
Guo, Linlin [1 ]
Chen, Jiabao [1 ]
Liu, Dong [1 ]
Liu, Lili [1 ]
机构：
[1] Jinzhou Med Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, 2,Sect 5,Renming St, Jinnzhou, Liaoning, Peoples R China
来源：
CANCER MANAGEMENT AND RESEARCH | 2020年 / 12卷
关键词：
OIP5-AS1; miR-137; ZNF217; epithelial ovarian cancer; METASTASIS; PROGNOSIS;
D O I：
10.2147/CMAR.S237726
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Long non-coding RNAs (lncRNAs) have been reported to play crucial regulatory roles in cellular activities and are associated with the carcinogenesis of various diseases. OIP5-AS1, as a novel lncRNA, function in epithelial ovarian cancer (EOC) still remains unclear. Material and Methods: qRT-PCR and Western blot analyses were performed to measure relevant expression, as needed. A series of functional experiments were performed to determine the role of OIP5-AS1 in EOC cells. Luciferase report, RNA pull down and RIP assays were performed to testify the interaction between relevant RNAs. Results: We found that OIP5-AS1 was significantly overexpressed in EOC. Knockdown of OIP5-AS1 inhibited cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process, yet facilitated apoptosis in vitro. OIP5-AS1 functioned as a competing endogenous RNA (ceRNA) to elevate ZNF217 expression through sponging miR-137. Furthermore, miR-137 inhibition and ZNF217 upregulation can reverse the effects of silencing OIP5-AS1 on the cellular activities of ovarian cancer cells. Also, depleted OIP5-AS1 hindered tumor growth and metastasis in vivo. Conclusion: OIP5-AS1 regulated ovarian cancer progression via modulating miR-137/ZNF217 signaling, suggesting that targeting OIP5-AS1 could be conducive to EOC clinical treatment.
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收藏
页码：6707 / 6717
页数：11
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