An immunohistochemical study of the effects of orexin receptor blockade on phospholipase C-β3 level in rat hippocampal dentate gyrus neurons

被引：3

作者：

Mousavi, Zeinab ^{[1
]}

Kourosh-Arami, Masoumeh ^{[1
]}

Mohsenzadegan, Monireh ^{[2
]}

Komaki, Alireza ^{[3
]}

机构：

[1] Iran Univ Med Sci, Sch Adv Technol Med, Dept Neurosci, Tehran, Iran

[2] Iran Univ Med Sci, Allied Med Coll, Tehran, Iran

[3] Hamadan Univ Med Sci, Neurophysiol Res Ctr, Hamadan, Iran

来源：

BIOTECHNIC & HISTOCHEMISTRY | 2021年 / 96卷 / 03期

关键词：

Dentate gyrus; hippocampus; orexin; phospholipase C; rats; SB-334867; ACTIVATION; SYSTEM; CA1;

D O I：

10.1080/10520295.2020.1778088

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Orexin-A (hypocretin-1) is a neuropeptide that is produced in the lateral hypothalamic area (LHA) and promotes widespread cortical activation. We investigated the effect of SB-334867, a selective orexin receptor 1 (OXR1) antagonist, on phospholipase C-beta 3 (PLC beta 3) level using slices of rat hippocampus preparations and immunohistochemistry. We used three Wistar rats in each of three groups. The control group was untreated rats and SB vehicle and SB groups received SB vehicle and 10 mg/kg SB-334867 daily from postnatal day (PND) 12 to PND30, respectively. We found that the orexin receptor antagonist decreased the PLC beta 3 level in the inner and outer blades of dentate gyrus (DG) compared to SB vehicle treated rats. Orexin may increase the PLC beta 3 level in most regions of the rat hippocampus.

引用

页码：191 / 196

页数：6

共 28 条

[1] Role of orexin type-1 receptors in paragiganto-coerulear modulation of opioid withdrawal and tolerance: A site specific focus [J].

Ahmadi-Soleimani, S. Mohammad ;

Azizi, Hossein ;

Gompf, Heinrich S. ;

Semnanian, Saeed .

NEUROPHARMACOLOGY, 2017, 126 :25-37

[2]

Allan AM, 1997, ALCOHOL CLIN EXP RES, V21, P1534

[3]

Arami MK, 2011, PHYSIOL PHARMACOL, V14, P337

[4] Postnatal development changes in excitatory synaptic activity in the rat locus coeruleus neurons [J].

Arami, Masoumeh Kourosh ;

Hajizadeh, Sohrab ;

Semnanian, Saeed .

BRAIN RESEARCH, 2016, 1648 :365-371

[5]

Arami MK, 2015, IRAN J BASIC MED SCI, V18, P989

[6]

Arami MK, 2005, IRAN J DIABETES LIPI, V4, pE2+E2i

[7] Alteration of Phospholipase C Expression in Rat Visual Cortical Neurons by Chronic Blockade of Orexin Receptor 1 [J].

Babasafari, Mahdieh ;

Kourosharami, Masoumeh ;

Behman, Jamie ;

Farhadi, Mona ;

Komaki, Alireza .

INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, 2020, 26 (03) :1485-1491

[8] Biophysical and phenomenological models of multiple spike interactions in spike-timing dependent plasticity [J].

Badoual, Mathilde ;

Zou, Quan ;

Davison, Andrew P. ;

Rudolph, Michael ;

Bal, Thierry ;

Fregnac, Yves ;

Destexhe, Alain .

INTERNATIONAL JOURNAL OF NEURAL SYSTEMS, 2006, 16 (02) :79-97

[9] Orexin-A Increases the Firing Activity of Hippocampal CA1 Neurons Through Orexin-1 Receptors [J].

Chen, Xin-Yi ;

Chen, Lei ;

Du, Yi-Feng .

JOURNAL OF NEUROSCIENCE RESEARCH, 2017, 95 (07) :1415-1426

[10] Orexin Depolarizes Central Amygdala Neurons via Orexin Receptor 1, Phospholipase C and Sodium-Calcium Exchanger and Modulates Conditioned Fear [J].

Dustrude, Erik T. ;

Caliman, Izabela F. ;

Bernabe, Cristian S. ;

Fitz, Stephanie D. ;

Grafe, Laura A. ;

Bhatnagar, Seema ;

Bonaventure, Pascal ;

Johnson, Philip L. ;

Molosh, Andrei, I ;

Shekhar, Anantha .

FRONTIERS IN NEUROSCIENCE, 2018, 12

← 1 2 3 →