Crystallization of high-quality protein crystals using an external electric field

被引：20

作者：

Koizumi, H. ^{[1
]}

Uda, S. ^{[1
]}

Fujiwara, K. ^{[1
]}

Tachibana, M. ^{[2
]}

Kojima, K. ^{[3
]}

Nozawa, J. ^{[1
]}

机构：

[1] Tohoku Univ, Inst Mat Res, Aoba Ku, Sendai, Miyagi 9808577, Japan

[2] Yokohama City Univ, Grad Sch Nanobiosci, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan

[3] Yokohama Soei Univ, Dept Educ, Midori Ku, Yokohama, Kanagawa 2260015, Japan

来源：

JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2015年 / 48卷

关键词：

protein crystallization; external electric fields; WHITE LYSOZYME CRYSTALS; X-RAY-DIFFRACTION; PROSTAGLANDIN D SYNTHASE; GEL-ACUPUNCTURE METHOD; MAGNETIC-FIELD; NUCLEATION RATE; DIELECTRIC-PROPERTIES; CYTOCHROME-C; GROWTH; MICROGRAVITY;

D O I：

10.1107/S1600576715015885

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The effect of a 20 kHz external electric field on the quality of tetragonal hen egg white (HEW) lysozyme crystals was investigated using X-ray diffraction rocking-curve measurements. The full width at half-maximum was found to be larger for high-order reflections but smaller for low-order reflections. In particular, it was revealed that a large amount of local strain is accumulated in tetragonal HEW lysozyme crystals grown under an applied field at 20 kHz. Comparison with previous results obtained for crystals grown with an applied field at 1 MHz [Koizumi, Uda, Fujiwara, Tachibana, Kojima & Nozawa (2013). J. Appl. Cryst. 46, 25-29] indicated that improvement of the protein crystal quality could be achieved by selection of an appropriate frequency for the applied electric field, which has a significant effect on the growth of the solid.

引用

页码：1507 / 1513

页数：7

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