Effects of Hg2+ on Luciferase mRNA Expression and Bioluminescence of Photobacterium leiognathi

被引：0

作者：

Li, Ming ^{[1
]}

Wang, Jingxue ^{[1
]}

Lin, Hong ^{[1
]}

机构：

[1] Ocean Univ China, Food Safety Lab, Qingdao 266003, Peoples R China

来源：

ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 1 | 2011年

关键词：

He2+; luciferase; gene expression; bioluminescence; Semi-quantitative RT-PCR; BACTERIAL BIOLUMINESCENCE; INHIBITION; LUMINESCENCE;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Objectives The aim of this study was to investigate the effects of Hg2+ on the mRNA expression of luciferase genes, as well as on the emission activity of the bioluminescence enzyme system. Methods Hg2+ was added into culture medium of Photobacterium leiognathi to detecte gene expression of luciferase by semi-quantitative RT-PCR, and was mixed with extracted enzymes to detecte the inhibitory effects of Hg2+ on the bioluminescence system. Results Expression of luciferase mRNA in the experimental group (0.83 <= 0.13) was significantly higher (P<0.01) than that in the control group (0.45 +/- 0.01) at the beginning of the log phase. The toxic effect on the bioluminescence system of enzymes increased proportionately with the concentration of Hg2+. Conclusion Hg2+ in low concentration (<= 0.5mg/L) can induce the expression of lux genes during the early stages of log phase, but it inhibits the intensity of the bioluminescence system of enzymes.

引用

页码：216 / 221

页数：6

共 17 条

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