Effect of the Combination of Training and ERRα Inhibition on Liver Metabolism by Modulation of PDK4 and LXR-α Expression in STZ-Induced Diabetic and Healthy Rats

Diabetes is a public health problem that affects life quality. Exercise training (ET) and controlled dietary habits improve metabolic diseases such as diabetes. The mechanisms by which exercise training ameliorate metabolic diseases are not fully clear. We designed the current study to evaluate the combination of ERR alpha suppression and ET effects on the expression of LXR-alpha, PDK4, and PPAR alpha in healthy and STZ-induced diabetic rats. Fifty-six male Wistar rats were divided into 8 groups(n = 7) as follows: control. diabetic control (a single dose of 45 mg/kg of STZ). ERR alpha inhibition group(received 0.48 mg/kg of XCT790). endurance training, diabetic rats which received XCT790 diabetic rats which performed endurance training, rats which received XCT790 and performed endurance training, and diabetic rats which received XCT790 and also performed endurance training. Expression of the target gene and protein was carried out on the lip er tissue. Our results shoved that ET significantly increased PDK4. PPAR alpha and ERR alpha expression. ERR alpha suppression significantly increased LXR-alpha and PDK4 expression in healthy rats compared to the healthy control group. In the diabetic group with ERR alpha suppression LXR-alpha expression significantly upregulated. The combination of ET and ERR alpha suppression did not change LXR-alpha expression compared to healthy and diabetic groups (CTL/ERR), but the expression of PDK4 PPAR alpha, and ERR alpha was significantly upregulated.