Detection of recombinant chymosins in calf rennet by enzyme-linked immunosorbent assay

被引：0

作者：

Collin, JC ^{[1
]}

Moulins, I ^{[1
]}

RoletRepecaud, O ^{[1
]}

Bailly, C ^{[1
]}

Lagarde, G ^{[1
]}

机构：

[1] SYNDICAT PROFESS PROD AUXILIAIRES IND LAITIERE,F-75001 PARIS,FRANCE

来源：

LAIT | 1997年 / 77卷 / 03期

关键词：

recombinant chymosin; detection; identification; ELISA; calf rennet;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The presence and the origin of recombinant chymosin can be detected in milk-clotting enzyme solutions by antigen coat plate-enzyme-linked immunosorbent assay (ACP-ELISA) or sandwich ELISA. The method is based on the detection of contaminant proteins derived from microorganisms or from the culture medium, which are always present in fermentation-produced chymosin solutions. The specific polyclonal antibodies obtained from the sera cf rabbit and hen permitted identification of the contaminant proteins in the commercial solutions. A simple dilution of the sample is sufficient to detect the addition of 0.5% Chymogen (Hansen) or Maxiren (Gist-Brocades) and 2.5% Chymax (Pfizer) in calf rennet.

引用

页码：425 / 431

页数：7

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