Development and application of high-resolution melting analysis for the classification of infectious laryngotracheitis virus strains and detection of recombinant progeny

被引:10
作者
Fakhri, Omid [1 ]
Hartley, Carol A. [1 ]
Devlin, Joanne M. [1 ]
Browning, Glenn F. [1 ]
Noormohammadi, Amir H. [2 ]
Lee, Sang-Won [1 ,3 ]
机构
[1] Univ Melbourne, Fac Vet & Agr Sci, Asia Pacific Ctr Anim Hlth, Parkville, Vic, Australia
[2] Univ Melbourne, Fac Vet & Agr Sci, Asia Pacific Ctr Anim Hlth, Werribee, Vic, Australia
[3] Konkuk Univ, Coll Vet Med, Seoul, South Korea
基金
澳大利亚研究理事会;
关键词
POLYMERASE-CHAIN-REACTION; RESTRICTION-ENDONUCLEASE ANALYSIS; LIVE ATTENUATED VACCINES; UNITED-STATES; RAPID DETECTION; DIFFERENTIATION; POLYMORPHISM; IDENTIFICATION; SEQUENCES; CHICKENS;
D O I
10.1007/s00705-018-4086-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Live attenuated vaccines against infectious laryngotracheitis virus (ILTV) are widely used in the poultry industry to control disease and help prevent economic losses. Molecular epidemiological studies of currently circulating strains of ILTV within poultry flocks in Australia have demonstrated the presence of highly virulent viruses generated by genomic recombination events between vaccine strains. In this study, high-resolution melting (HRM) analysis was used to develop a tool to classify ILTV isolates and to investigate ILTV recombination. The assay was applied to plaque-purified progeny viruses generated after co-infection of chicken embryo kidney (CEK) monolayers with the A20 and Serva ILT vaccine strains and also to viruses isolated from field samples. The results showed that the HRM analysis is a suitable tool for the classification of ILTV isolates and can be used to detect recombination between ILTV vaccine strains in vitro. This method can be used to classify a broad range of ILTV strains to facilitate the classification and genotyping of ILTV and help to further understand recombination in these viruses.
引用
收藏
页码:427 / 438
页数:12
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