Cell shape dynamics during the staphylococcal cell cycle

被引：181

作者：

Monteiro, Joao M. ^{[1
]}

Fernandes, Pedro B. ^{[1
]}

Vaz, Filipa ^{[2
]}

Pereira, Ana R. ^{[1
]}

Tavares, Andreia C. ^{[1
]}

Ferreira, Maria T. ^{[1
]}

Pereira, Pedro M. ^{[1
]}

Veiga, Helena ^{[1
]}

Kuru, Erkin ^{[3
,4
]}

VanNieuwenhze, Michael S. ^{[3
]}

Brun, Yves V. ^{[4
]}

Filipe, Sergio R. ^{[2
]}

Pinho, Mariana G. ^{[1
]}

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Lab Bacterial Cell Biol, P-2780157 Oeiras, Portugal

[2] Univ Nova Lisboa, Inst Tecnol Quim & Biol Antonio Xavier, Lab Bacterial Cell Surfaces & Pathogenesis, P-2780157 Oeiras, Portugal

[3] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[4] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

来源：

NATURE COMMUNICATIONS | 2015年 / 6卷

基金：

欧洲研究理事会;

关键词：

L-ALANINE AMIDASE; BETA-N-ACETYLGLUCOSAMINIDASE; PEPTIDOGLYCAN CROSS-LINKING; GRAM-POSITIVE BACTERIA; D-AMINO ACIDS; FLUORESCENCE MICROSCOPY; BACILLUS-SUBTILIS; WALL SYNTHESIS; IN-SITU; AUREUS;

D O I：

10.1038/ncomms9055

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Staphylococcus aureus is an aggressive pathogen and a model organism to study cell division in sequential orthogonal planes in spherical bacteria. However, the small size of staphylococcal cells has impaired analysis of changes in morphology during the cell cycle. Here we use super-resolution microscopy and determine that S. aureus cells are not spherical throughout the cell cycle, but elongate during specific time windows, through peptidoglycan synthesis and remodelling. Both peptidoglycan hydrolysis and turgor pressure are required during division for reshaping the flat division septum into a curved surface. In this process, the septum generates less than one hemisphere of each daughter cell, a trait we show is common to other cocci. Therefore, cell surface scars of previous divisions do not divide the cells in quadrants, generating asymmetry in the daughter cells. Our results introduce a need to reassess the models for division plane selection in cocci.

引用

页数：12

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