Triggered isothermal PCR by denaturation bubble-mediated strand exchange amplification

被引：77

作者：

Shi, Chao ^{[1
]}

Shang, Fanjin ^{[2
]}

Zhou, Meiling ^{[2
]}

Zhang, Pansong ^{[3
]}

Wang, Yifan ^{[2
]}

Ma, Cuiping ^{[2
]}

机构：

[1] Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China

[2] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China

[3] Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 77期

基金：

中国国家自然科学基金;

关键词：

DNA-POLYMERASE; CARE; FLUCTUATIONS; DIAGNOSTICS; POINT;

D O I：

10.1039/c6cc05906f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Here, we introduced the concept of strand exchange amplification (SEA) mediated by denaturation bubbles. Similar to traditional PCR, it only employed a DNA polymerase and a pair of common primers to realize a three-step cycle process, but the entire SEA reaction was performed at a single temperature.

引用

页码：11551 / 11554

页数：4

共 32 条

[1]

Altan-Bonnet G, 2003, PHYS REV LETT, V90, DOI [10.1103/PhysRevLett.90.138101, 10.1103/PhysRevLEtt.90.138101]

[2]

Craw P, 2012, LAB CHIP, V12, P2469, DOI [10.1039/c21c40100b, 10.1039/c2lc40100b]

[3] In vitro reconstitution of the late steps of genetic recombination in E-coli [J].

Eggleston, AK ;

Mitchell, AH ;

West, SC .

CELL, 1997, 89 (04) :607-617

[4] Watching DNA breath one molecule at a time [J].

Fei, Jingyi ;

Ha, Taekjip .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (43) :17173-17174

[5] Fluctuations in the DNA double helix: A critical review [J].

Frank-Kamenetskii, Maxim D. ;

Prakash, Shikha .

PHYSICS OF LIFE REVIEWS, 2014, 11 (02) :153-170

[6] DNA damage and repair [J].

Friedberg, EC .

NATURE, 2003, 421 (6921) :436-440

[7] Bubble dynamics in DNA [J].

Hanke, A ;

Metzler, R .

JOURNAL OF PHYSICS A-MATHEMATICAL AND GENERAL, 2003, 36 (36) :L473-L480

[8] Rapid, Sensitive, and Quantitative Detection of Pathogenic DNA at the Point of Care through Microfluidic Electrochemical Quantitative Loop-Mediated Isothermal Amplification [J].

Hsieh, Kuangwen ;

Patterson, Adriana S. ;

Ferguson, B. Scott ;

Plaxco, Kevin W. ;

Soh, H. Tom .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (20) :4896-4900

[9] Supercoiling Induces Denaturation Bubbles in Circular DNA [J].

Jeon, Jae-Hyung ;

Adamcik, Jozef ;

Dietler, Giovanni ;

Metzler, Ralf .

PHYSICAL REVIEW LETTERS, 2010, 105 (20)

[10] Breathing fluctuations in position-specific DNA base pairs are involved in regulating helicase movement into the replication fork [J].

Jose, Davis ;

Weitzel, Steven E. ;

von Hippel, Peter H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (36) :14428-14433

← 1 2 3 4 →