Hemopexin is up-regulated in plasma from type 1 diabetes mellitus patients: Role of glucose-induced ROS

被引：21

作者：

Chen, Chia-Ching ^{[2
]}

Lu, Ying-Chieh ^{[3
,4
]}

Chen, Yi-Wen ^{[3
,4
]}

Lee, Wen-Li ^{[2
]}

Lu, Chieh-Hsiang ^{[5
]}

Chen, You-Hsuan ^{[3
,4
]}

Lee, Yun-Ching ^{[3
,4
]}

Lin, Szu-Ting ^{[3
,4
]}

Timms, John F. ^{[1
]}

Lee, Ying-Ray ^{[6
,7
]}

Chou, Hsiu-Chuan ^{[8
]}

Chan, Hong-Lin ^{[3
,4
]}

机构：

[1] UCL, EGA Inst Womens Hlth, London, England

[2] Chiayi Christian Hosp, Dept Pediat, Chiayi, Taiwan

[3] Natl Tsing Hua Univ, Inst Bioinformat & Struct Biol, Hsinchu, Taiwan

[4] Natl Tsing Hua Univ, Dept Med Sci, Hsinchu, Taiwan

[5] Chiayi Chirsitan Hosp, Div Endocrinol & Metab, Dept Internal Med, Chiayi, Taiwan

[6] Chiayi Chirsitan Hosp, Dept Med Res, Chiayi, Taiwan

[7] Min Hwei Coll Hlth Care Management, Tainan, Taiwan

[8] Natl Hsinchu Univ Educ, Dept Appl Sci, Hsinchu, Taiwan

来源：

JOURNAL OF PROTEOMICS | 2012年 / 75卷 / 12期

关键词：

Type 1 diabetes mellitus; 2D-DIGE; MALDI-TOF mass spectrometry; Reactive oxygen species; Hemopexin; DIFFERENCE GEL-ELECTROPHORESIS; OXIDATIVE STRESS; PROTEOMIC ANALYSIS; CARDIOVASCULAR-DISEASE; ENDOTHELIAL-CELLS; PROTEIN GLYCATION; INDUCED APOPTOSIS; EPITHELIAL-CELLS; GENE-EXPRESSION; ANGIOGENESIS;

D O I：

10.1016/j.jprot.2012.04.047

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Type 1 diabetes mellitus (T1DM) is an insulin-dependent metabolic disease in the world and often occurs in children and adolescents. Recent advances in quantitative proteomics offer potential for the discovery of plasma proteins as biomarkers for tracking disease progression and for understanding the molecular mechanisms of diabetes. Comparative proteomic analysis of the plasma proteomes from T1DM cases and healthy donors with lysine- and cysteine-labeling 2D-DIGE combining MALDI-TOF/TOF mass spectrometry revealed that 39 identified T1DM-associated plasma proteins showed significant changes in protein expression including hemopexin, and 41 in thiol reactivity. Further study showed that hemopexin can be induced in numerous cell lines by increasing the glucose concentration in the medium. Interestingly, glucose-induced hemopexin expression can be reduced by reactive oxygen species (ROS) scavengers such as glutathione, implying that hemopexin expression is linked to glucose-induced oxidative stress. In conclusion, the current work has identified potential T1DM biomarkers and one of these, hemopexin, can be modulated by glucose through a ROS-dependent mechanism. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：3760 / 3777

页数：18

共 54 条

[1] Effect of diabetes mellitus on formation of coronary collateral vessels [J].