Detection of carbon monoxide from biological tissue using difference frequency generation in periodically-poled lithium niobate near 4.6 μm

被引：0

作者：

Morimoto, Y ^{[1
]}

Klattenhoff, J ^{[1
]}

Durante, W ^{[1
]}

Lancaster, DG ^{[1
]}

Curl, RF ^{[1
]}

Tittel, FK ^{[1
]}

机构：

[1] Rice Univ, Rice Quantum Inst, Houston, TX 77251 USA

来源：

SYSTEMS AND TECHNOLOGIES FOR CLINICAL DIAGNOSTICS AND DRUG DISCOVERY II, PROCEEDINGS OF | 1999年 / 3603卷

关键词：

gas detection; laser spectroscopy; vascular smooth muscle cells;

D O I：

10.1117/12.346754

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A mid-infrared gas sensor using difference frequency generation was developed to measure trace levels (similar to ppb) of biological carbon monoxide (CO). A periodically-poled lithium niobate crystal is pumped by a continuous wave Ti: Sapphire laser and a compact diode pumped Nd:YAG laser operating at 864.86 and 1064.6 nm, respectively. The strong infrared transition R(6) at 2169.2 cm(-1)(4.61 mu m) is chosen for convenient CO detection without interference from other gas species. Carbon monoxide is collected and flowed into a multipass cell with an effective optical path length of 18.3 m. Using such an experimental arrangement, we detected the generation of CO at levels of 30 ppb during a 30 min period from living vascular smooth muscle cells (1 x 10(7)) in basal state.

引用

页码：30 / 35

页数：6