LytA, Major Autolysin of Streptococcus pneumoniae, Requires Access to Nascent Peptidoglycan

被引：107

作者：

Mellroth, Peter ^{[1
]}

Daniels, Robert ^{[2
]}

Eberhardt, Alice ^{[1
]}

Ronnlund, Daniel ^{[3
]}

Blom, Hans ^{[3
]}

Widengren, Jerker ^{[3
]}

Normark, Staffan ^{[1
]}

Henriques-Normark, Birgitta ^{[1
,4
]}

机构：

[1] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17177 Stockholm, Sweden

[2] Stockholm Univ, Dept Biochem & Biophys, Ctr Biomembrane Res, S-10691 Stockholm, Sweden

[3] Albanova Univ Ctr, Royal Inst Technol, Dept Appl Phys, S-10691 Stockholm, Sweden

[4] Karolinska Univ Hosp, Dept Lab Med, Div Clin Microbiol, S-17176 Stockholm, Sweden

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2012年 / 287卷 / 14期

基金：

瑞典研究理事会;

关键词：

PENICILLIN RESISTANCE; PNEUMOCOCCAL AUTOLYSIN; RECOGNITION PROTEIN; SURFACE-PROTEINS; ENZYME-ACTIVITY; PNEUMOLYSIN; FRATRICIDE; VIRULENCE; GENE; ATTENUATION;

D O I：

10.1074/jbc.M111.318584

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: The regulation of cell wall hydrolysis by the pneumococcal autolysin LytA is poorly understood. Results: The cell wall is susceptible to extracellular LytA only during the stationary phase or after cell wall synthesis inhibition. Conclusion: LytA is regulated on the substrate level, where peptidoglycan modifications likely prevent LytA hydrolysis. Significance: The control of amidases is essential for bacterial survival, cell-wall synthesis, and division.

引用

页码：11018 / 11029

页数：12

共 38 条

[1] Studies on the enzymes of pneumococcus. IV. Bacteriolytic enzyme. [J].

Avery, OT ;

Cullen, GE .

JOURNAL OF EXPERIMENTAL MEDICINE, 1923, 38 (02) :199-206

[2] Additive attenuation of virulence of Streptococcus pneumoniae by mutation of the genes encoding pneumolysin and other putative pneumococcal virulence proteins [J].

Berry, AM ;

Paton, JC .

INFECTION AND IMMUNITY, 2000, 68 (01) :133-140

[3] THE ROLE OF PNEUMOLYSIN AND AUTOLYSIN IN THE PATHOLOGY OF PNEUMONIA AND SEPTICEMIA IN MICE INFECTED WITH A TYPE-2 PNEUMOCOCCUS [J].

CANVIN, JR ;

MARVIN, AP ;

SIVAKUMARAN, M ;

PATON, JC ;

BOULNOIS, GJ ;

ANDREW, PW ;

MITCHELL, TJ .

JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (01) :119-123

[4] Signal sequence directs localized secretion of bacterial surface proteins [J].

Carlsson, Fredric ;

Stalhammar-Carlemalm, Margaretha ;

Flaerdh, Klas ;

Sandin, Charlotta ;

Carlemalm, Eric ;

Lindahl, Gunnar .

NATURE, 2006, 442 (7105) :943-946

[5] Competence-induced fratricide in streptococci [J].

Claverys, Jean-Pierre ;

Martin, Bernard ;

Havarstein, Leiv Sigve .

MOLECULAR MICROBIOLOGY, 2007, 64 (06) :1423-1433

[6] Attenuation of penicillin resistance in a peptidoglycan O-acetyl transferase mutant of Streptococcus pneumoniae [J].

Crisostomo, M. Ines ;

Vollmer, Waldemar ;

Kharat, Arun S. ;

Inhuelsen, Silja ;

Gehre, Florian ;

Buckenmaier, Stephan ;

Tomasz, Alexander .

MOLECULAR MICROBIOLOGY, 2006, 61 (06) :1497-1509

[7] Disulfide Bond Formation and Cysteine Exclusion in Gram-positive Bacteria [J].

Daniels, Robert ;

Mellroth, Peter ;

Bernsel, Andreas ;

Neiers, Fabrice ;

Normark, Staffan ;

von Heijne, Gunnar ;

Henriques-Normark, Birgitta .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (05) :3300-3309

[8] Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus [J].

DeDent, Andrea ;

Bae, Taeok ;

Missiakas, Dominique M. ;

Schneewind, Olaf .

EMBO JOURNAL, 2008, 27 (20) :2656-2668

[9] Fratricide in Streptococcus pneumoniae: contributions and role of the cell wall hydrolases CbpD, LytA and LytC [J].

Eldholm, Vegard ;

Johnsborg, Ola ;

Haugen, Kristine ;

Ohnstad, Hilde Solheim ;

Havarstein, Leiv Sigve .

MICROBIOLOGY-SGM, 2009, 155 :2223-2234

[10] A novel solenoid fold in the cell wall anchoring domain of the pneumococcal virulence factor LytA [J].

Fernández-Tornero, C ;

López, R ;

García, E ;

Giménez-Gallego, G ;

Romero, A .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (12) :1020-1024

← 1 2 3 4 →