Galactose-Functionalized Gelatin Hydrogels Improve the Functionality of Encapsulated Hepg2 Cells

被引：20
作者：
Gevaert, Elien [1 ]
Billiet, Thomas [2 ]
Declercq, Heidi [1 ]
Dubruel, Peter [2 ]
Cornelissen, Ria [1 ]
机构：
[1] Univ Ghent, Tissue Engn Grp, B-9000 Ghent, Belgium
[2] Univ Ghent, Polymer Chem & Biomat Res Grp, B-9000 Ghent, Belgium
来源：
MACROMOLECULAR BIOSCIENCE | 2014年 / 14卷 / 03期
关键词：
biocompatibility; galactose; gelatin; hepatocyte; hydrogels; SYNTHETIC GLYCOPOLYMER MATRIX; BIOARTIFICIAL LIVER; HEPATOCYTE CULTURE; CARRYING POLYMERS; TRANSPLANTATION; EXPRESSION; ALGINATE; FUTURE;
D O I：
10.1002/mabi.201300320
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study investigates the effect of galactosylated gelatin on encapsulated HepG2 cells. Methacrylamide modified gelatin is evaluated and compared with its galactosylated counterpart with respect to effects on viability, morphological characteristics, proliferation, and the expression of hepatocyte specific markers. The research reveals that further modifications of methacrylamide modified gelatin are possible without affecting the survival of the encapsulated cells (viability of 90%). Moreover, the study demonstrates a clear and long-term (up to 21 d) improvement in hepatocyte specific gene expression when the cells are encapsulated in the galactosylated gelatin. It is concluded that the use of galactosylated gelatin derivates supports the hepatocyte phenotype.
引用
收藏
页码：419 / 427
页数：9
相关论文
共 35 条
[1]   Directed 3D cell alignment and elongation in microengineered hydrogels [J].
Aubin, Hug ;
Nichol, Jason W. ;
Hutson, Che B. ;
Bae, Hojae ;
Sieminski, Alisha L. ;
Cropek, Donald M. ;
Akhyari, Payam ;
Khademhosseini, Ali .
BIOMATERIALS, 2010, 31 (27) :6941-6951
[2]   Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds [J].
Balakrishnan, B ;
Jayakrishnan, A .
BIOMATERIALS, 2005, 26 (18) :3941-3951
[3]   CELL CELL AND CELL MATRIX INTERACTIONS DIFFERENTIALLY REGULATE THE EXPRESSION OF HEPATIC AND CYTOSKELETAL GENES IN PRIMARY CULTURES OF RAT HEPATOCYTES [J].
BENZEEV, A ;
ROBINSON, GS ;
BUCHER, NLR ;
FARMER, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2161-2165
[4]   Quantitative Contrasts in the Photopolymerization of Acrylamide and Methacrylamide-Functionalized Gelatin Hydrogel Building Blocks [J].
Billiet, Thomas ;
Van Gasse, Bjorn ;
Gevaert, Elien ;
Cornelissen, Maria ;
Martins, Jose C. ;
Dubruel, Peter .
MACROMOLECULAR BIOSCIENCE, 2013, 13 (11) :1531-1545
[5]   Future of bioartificial liver support [J].
Chamuleau, Robert A. F. M. .
WORLD JOURNAL OF GASTROINTESTINAL SURGERY, 2009, 1 (01) :21-25
[6]   Monolayer and Spheroid Culture of Human Liver Hepatocellular Carcinoma Cell Line Cells Demonstrate Distinct Global Gene Expression Patterns and Functional Phenotypes [J].
Chang, Tammy T. ;
Hughes-Fulford, Millie .
TISSUE ENGINEERING PART A, 2009, 15 (03) :559-567
[7]   Regulation of hepatocyte behaviors by galactose-carrying polymers through receptor-mediated mechanism [J].
Cho, C. S. ;
Hoshiba, T. ;
Harada, I. ;
Akaike, T. .
REACTIVE & FUNCTIONAL POLYMERS, 2007, 67 (11) :1301-1310
[8]   Galactose-carrying polymers as extracellular matrices for liver tissue engineering [J].
Cho, CS ;
Seo, SJ ;
Park, IK ;
Kim, SH ;
Kim, TH ;
Hoshiba, T ;
Harada, I ;
Akaike, T .
BIOMATERIALS, 2006, 27 (04) :576-585
[9]   In vitro evaluation of a multi-layer radial-flow bioreactor based on galactosylated chitosan nanofiber scaffolds [J].
Chu, Xue-Hui ;
Shi, Xiao-Lei ;
Feng, Zhang-Qi ;
Gu, Jin-Yang ;
Xu, Hai-Yun ;
Zhang, Yue ;
Gu, Zhong-Ze ;
Ding, Yi-Tao .
BIOMATERIALS, 2009, 30 (27) :4533-4538
[10]   Porous gelatin hydrogels: 2. In vitro cell interaction study [J].
Dubruel, Peter ;
Unger, R. ;
Van Vlierberghe, Sandra ;
Cnudde, Veerle ;
Jacobs, Patric J. S. ;
Schacht, Etienne ;
Kirkpatrick, C. J. .
BIOMACROMOLECULES, 2007, 8 (02) :338-344
1234