High resolution Y chromosome typing: 19 STRs amplified in three multiplex reactions

被引：83
作者：
Bosch, E
Lee, AC
Calafell, F
Arroyo, E
Henneman, P
de Knijff, P
Jobling, MA
机构：
[1] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England
[2] Univ Pompeu Fabra, Fac Ciencies Salut & Vida, Unitat Biol Evolut, Barcelona, Spain
[3] Univ Complutense Madrid, Fac Med, Madrid, Spain
[4] Leiden Univ, Forens Lab DNA Res, MGC Dept Human & Clin Genet, Ctr Med, Leiden, Netherlands
来源：
FORENSIC SCIENCE INTERNATIONAL | 2002年 / 125卷 / 01期
基金：
英国惠康基金;
关键词：
Y chromosome; STR polymorphisms; microsatellites; haplotypes; Iberian Peninsula; multiplex;
D O I：
10.1016/S0379-0738(01)00627-2
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
Nineteen Y-specific short tandem repeat (STR) loci have been amplified in 768 samples from the Iberian Peninsula in order to evaluate their usefulness in forensic casework. Two previously published multiplex reactions by Thomas Ct al. [Hum. Genet. 6 (1999)577] (MS 1, modified here: DYS19,DYS388, DYS390, DYS391, DYS392 and DYS393)and by Ayub et al. [Nucl. Acids Res. 28 (2000) e8] (CTS: DYS434, DYS435, DYS436, DYS437, DYS438 and DYS439) plus a novel one reported here (EBF: DYS385, DYS389, DYS460, DYS461, DYS462 and amelogenin) have been used. DYS385, DYS439 and DYS391 were the most informative loci with allele diversities of 0.7997, 0.6683 and 0.5940, respectively. A total of 635 different haplotypes were observed, of which 573 (90.24%) were found in single individuals. The overall haplotype diversity was 0.9988 and that obtained by each multiplex system was 0.9812 for EBF, 0.9292 for MS 1 and 0.9089 for CTS. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：42 / 51
页数：10
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