Field evidence of selenium bioreduction in a uranium-contaminated aquifer

被引：46

作者：

Williams, Kenneth H. ^{[1
]}

Wilkins, Michael J. ^{[2
]}

N'Guessan, A. Lucie ^{[2
]}

Arey, Bruce ^{[2
]}

Dodova, Elena ^{[3
]}

Dohnalkova, Alice ^{[2
]}

Holmes, Dawn ^{[3
]}

Lovley, Derek R. ^{[3
]}

Long, Philip E. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA

[2] Pacific NW Natl Lab, Richland, WA 99352 USA

[3] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA

来源：

ENVIRONMENTAL MICROBIOLOGY REPORTS | 2013年 / 5卷 / 03期

关键词：

MEMBRANE BIOFILM REACTOR; SELENATE REDUCTION; COMMUNITY STRUCTURE; BIOREMEDIATION; RESPIRATION; GROUNDWATER; METABOLISM; DECHLOROMONAS; SEDIMENTS; STRAIN;

D O I：

10.1111/1758-2229.12032

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Removal of selenium from groundwater was documented during injection of acetate into a uranium-contaminated aquifer near Rifle, Colorado (USA). Bioreduction of aqueous selenium to its elemental form (Se0) concentrated it within mineralized biofilms affixed to tubing used to circulate acetate-amended groundwater. Scanning and transmission electron microscopy revealed close association between Se0 precipitates and cell surfaces, with Se0 aggregates having a diameter of 5060nm. Accumulation of Se0 within biofilms occurred over a three-week interval at a rate of c. 9mg Se0m2 tubing day1. Removal was inferred to result from the activity of a mixed microbial community within the biofilms capable of coupling acetate oxidation to the reduction of oxygen, nitrate and selenate. Phylogenetic analysis of the biofilm revealed a community dominated by strains of Dechloromonas sp. and Thauera sp., with isolates exhibiting genetic similarity to the latter known to reduce selenate to Se0. Enrichment cultures of selenate-respiring microorganisms were readily established using Rifle site groundwater and acetate, with cultures dominated by strains closely related to D.aromatica (9699% similarity). Predominance of Dechloromonas sp. in recovered biofilms and enrichments suggests this microorganism may play a role in the removal of selenium oxyanions present in Se-impacted groundwaters and sediments.

引用

页码：444 / 452

页数：9

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