Odorant-binding proteins and olfactory coding in the solitary bee Osmia cornuta

被引：27
作者：
Yin, Xue-Wei [1 ,6 ]
Iovinella, Immacolata [1 ]
Marangoni, Roberto [2 ]
Cattonaro, Federica [3 ]
Flamini, Guido [4 ]
Sagona, Simona [5 ]
Zhang, Long [6 ]
Pelosi, Paolo [1 ]
Felicioli, Antonio [5 ]
机构：
[1] Univ Pisa, Dept Agr Food & Environm, Pisa, Italy
[2] Univ Pisa, Dept Biol, Pisa, Italy
[3] IGA Technol Serv Srl, Udine, Italy
[4] Univ Pisa, Dept Pharm, Pisa, Italy
[5] Univ Pisa, Dept Vet Sci, Pisa, Italy
[6] China Agr Univ, Minist Agr, Key Lab Biol Control, Beijing 100094, Peoples R China
来源：
CELLULAR AND MOLECULAR LIFE SCIENCES | 2013年 / 70卷 / 16期
关键词：
Osmia cornuta; Solitary bees; Chemical communication; Transcriptome; Odorant-binding proteins; Olfactory receptors; APIS-MELLIFERA; SOLUBLE-PROTEINS; HONEY-BEE; CHEMOSENSORY PROTEIN; PHEROMONE-BINDING; HYMENOPTERA-MEGACHILIDAE; NEST ESTABLISHMENT; LIGAND-BINDING; EXPRESSION; INSECT;
D O I：
10.1007/s00018-013-1308-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Solitary bees are major pollinators but their chemical communication system has been poorly studied. We investigated olfactory coding in Osmia cornuta from two perspectives, chemical and biochemical. We identified (E)-geranyl acetone and 2-hexyl-1,3-dioxolane, specifically secreted by females and males, respectively. A transcriptome analysis of antennae revealed 48 ORs (olfactory receptors), six OBPs (odorant-binding proteins), five CSPs (chemosensory proteins), and a single SNMP (sensory neuron membrane protein). The numbers of ORs and OBPs are much lower than in the honeybee, in particular, C-minus OBPs are lacking in the antennae of O. cornuta. We have expressed all six OBPs of O. cornuta and studied their binding specificities. The best ligands are common terpene plant odorants and both volatiles produced by the bee and identified in this work.
引用
收藏
页码：3029 / 3039
页数：11
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