A novel counter-selection method for markerless genetic modification in Synechocystis sp PCC 6803

被引：38

作者：

Cheah, Yi Ern ^{[1
]}

Albers, Stevan C. ^{[2
]}

Peebles, Christie A. M. ^{[1
,2
]}

机构：

[1] Colorado State Univ, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Cell & Mol Biol Grad Program, Ft Collins, CO 80523 USA

来源：

BIOTECHNOLOGY PROGRESS | 2013年 / 29卷 / 01期

关键词：

cyanobacteria; Synechocystis; homologous recombination; markerless transformation; genetic engineering; nickel response system; mazF; SELECTABLE MARKER; PHOTOSYSTEM-II; PROTEIN; MAZF; EXPRESSION;

D O I：

10.1002/btpr.1661

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The cyanobacterium Synechocystis sp. PCC 6803 is a photosynthetic organism capable of efficient harnessing of solar energy while capturing CO2 from the environment. Methods to genetically alter its genomic DNA are essential for elucidating gene functions and are useful tools for metabolic engineering. In this study, a novel counter-selection method for the genetic alteration of Synechocystis was developed. This method utilizes the nickel inducible expression of mazF, a general protein synthesis inhibitor, as a counter-selection marker. Counter-selection is particularly useful because the engineered strain is free of any markers which make further genetic modification independent of available antibiotic resistance genes. The usability of this method was further demonstrated by altering genes at several loci in two variants of Synechocystis. (c) 2012 American Institute of Chemical Engineers Biotechnol. Prog. 29;23-30, 2013

引用

页码：23 / 30

页数：8

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