Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength

被引：56

作者：

Liu, Helin ^{[1
,2
]}

Fan, Yanyan ^{[3
]}

Wang, Jianhai ^{[1
,2
]}

Song, Zhongsen ^{[3
]}

Shi, Hao ^{[3
]}

Han, Rongcheng ^{[3
]}

Sha, Yinlin ^{[1
,2
]}

Jiang, Yuqiang ^{[3
]}

机构：

[1] Peking Univ, Sch Basic Med Sci, Dept Biophys, Single Mol & Nanobiol Lab, Beijing 100191, Peoples R China

[2] Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

中国国家自然科学基金;

关键词：

QUANTUM DOTS; LIVING CELLS; THERMOMETER; PROBES; HEAT;

D O I：

10.1038/srep14879

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Luminescence thermometry usually suffer from cellular complexity of the biochemical environment (such as pH and ionic strength), and thus the accuracy and reliability of the determined intracellular temperature are directly affected. Herein, a photoluminescent nanothermometer composed of polymer encapsulated quantum dots (P-QD) has been developed. And the prepared nanothermometer exhibits some advantages: such as non-sensitivity to pH and ionic strength, as well as high detection sensitivity and ultrahigh reversibility. The intracellular temperature was accurately determined under physiological conditions with different pH and ionic strength, and direct measurement of thermogenesis in individual cells has been achieved.

引用

页数：8

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