Generation of expressed sequence tags for immune gene discovery and marker development in the sea squirt, Halocynthia roretzi

被引：0

作者：

Kim, Young-Ok ^{[1
]}

Cho, Hyun Kook ^{[2
]}

Park, Eun-Mi ^{[1
]}

Nam, Bo-Hye ^{[1
]}

Hur, Young Baek ^{[3
]}

Lee, Sang-Jun ^{[1
]}

Cheong, JaeHun ^{[2
]}

机构：

[1] Natl Fisheries Res & Dev Inst, Biotechnol Res Inst, Pusan 619902, South Korea

[2] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

[3] Res Ctr Aquaculture Environm, Tongyoung 650943, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2008年 / 18卷 / 09期

关键词：

sea squirt; Halocynthia roretzi; expressed sequence tags (ESTs); immune gene; marker;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expresssed sequence tag (EST) analysis was developed from three cDNA libraries constructed from cells of the digestive tract, gonad, and liver of sea squirt. Randomly selected cDNA clones were partially sequenced to generate a total of 922 ESTs, in which 687 unique ESTs were identified respectively. Results of BLASTX search showed that 612 ESTs (89%) have homology to genes of known function whereas 75 ESTs (11%) were unidentified or novel. Based on the major function of their encoded proteins, the identified clones were classified into ten broad categories. We also identified several kinds of immune-related genes as identifying novel genes. Sequence analysis of ESTs revealed the presence of microsatellite-containing genes that may be valuable for further gene mapping studies. The accumulation of a large number of identified cDNA clones is invaluable for the study of sea squirt genetics and developmental biology. Further studies using cDNA microarrays are needed to identify the differentially expressed transcripts after disease infection.

引用

页码：1510 / 1517

页数：8