Conjugating pyrene onto PNA-based fluorescent probes for improved detection selectivity toward double-stranded siRNA

被引：14

作者：

Sato, Yusuke ^{[1
]}

Takahashi, Yuki ^{[1
]}

Tanabe, Takaaki ^{[1
]}

Nishizawa, Seiichi ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Sci, Dept Chem, Sendai, Miyagi 9808578, Japan

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2020年 / 18卷 / 21期

基金：

日本学术振兴会;

关键词：

PEPTIDE NUCLEIC-ACID; TRIPLEX-FORMING PNA; THIAZOLE ORANGE; RNA; DELIVERY; PHARMACOKINETICS; BIODISTRIBUTION; RECOGNITION; SURROGATE; BINDING;

D O I：

10.1039/d0ob00794c

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We report on the design of new siRNA-binding fluorescent probes with the improved detection selectivity toward intact double-stranded siRNAs over single-stranded forms by the conjugation of pyrene unit into thiazole orange base surrogate-carrying peptide nucleic acid (PNA) that can simultaneously recognize the 3 '-overhang and double-stranded sequences of target siRNAs.

引用

收藏

页码：4009 / 4013

页数：5

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