Ketoconazole-loaded poly-(lactic acid) nanoparticles: Characterization and improvement of antifungal efficacy in vitro against Candida and dermatophytes

被引：24
作者：
Endo, E. H. [1 ]
Makimori, R. Y. [1 ]
Companhoni, M. V. P. [1 ]
Ueda-Nakamura, T. [2 ]
Nakamura, C., V [2 ]
Dias Filho, B. P. [2 ]
机构：
[1] Univ Estadual Maringa, Postgrad Program Pharmaceut Sci, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Dept Basic Hlth Sci, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil
来源：
JOURNAL DE MYCOLOGIE MEDICALE | 2020年 / 30卷 / 03期
关键词：
PLA; Nanoparticle; Ketoconazole; Candida sp; Biofilms; Dermatophytes; DRUG-DELIVERY SYSTEMS; PLA-NANOPARTICLES; POLY(LACTIC ACID); FORMULATION; OPTIMIZATION; RESISTANCE; SPP;
D O I：
10.1016/j.mycmed.2020.101003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Objective. - In order to improve the effect of ketoconazole, poly-lactic acid (PLA) nanoparticles containing ketoconazole were prepared, characterized and tested against dermatophytes and Candida spp planktonic and biofilm cells. Methods. - The ketoconazole-PLA nanoparticles obtained by nanoprecipitation were characterized using dynamic light scattering, scanning electron microscopy, transmission electron microscopy, and differential scanning calorimetry. In addition, quantification of encapsulated ketoconazole and the in vitro release profile were determined. Antifungal susceptibility tests against dermatophytes Trichophyton rubrum, Trichophyton mentagrophytes, and Microsporum gypseum and yeasts Candida albicans, C. dubliniensis, C. krusei, C. parapsilosis, and C. tropicalis were performed. Results. - Spherical nanoparticles, with a mean diameter of 188.5 nm and an encapsulation efficiency of 45% ketoconazole, were obtained. The nanoparticles containing ketoconazole had superior antifungal activity against all tested fungi strains than free ketoconazole. Inhibition of yeast biofilm formation was also achieved. Conclusion. - Ketoconazole-PLA nanoparticles resulted in better antifungal activity of ketoconazole nanoparticles than free drug against dermatophytes and Candida species, indicating a promising tool for the development of therapeutic strategies. (C) 2020 Elsevier Masson SAS. All rights reserved.
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页数：6
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