Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defect increased FGF-2, osteocalcin and OPG immunostaining and IL-10 levels

被引：0

作者：

Vasconcelos, Roseane Carvalho ^{[1
]}

Ferreira, Camyla ^{[2
]}

de Araujo, Eduarda Medeiros ^{[2
]}

Motta, Fabiana ^{[2
]}

Bomio, Mauricio ^{[2
]}

de Araujo Junior, Raimundo Fernandes ^{[3
]}

Fernandes Paiva, Daniel Felipe ^{[4
]}

Pirih, Flavia Q. ^{[5
]}

Pereira da Silva, Jose Sandro ^{[6
]}

Chan, Alan B. ^{[7
]}

Cruz, Luis J. ^{[8
]}

Ishii, Makiko ^{[9
]}

Carvalho Xavier de Medeiros, Caroline Addison ^{[10
]}

Bernardo Guerra, Gerlane Coelho ^{[11
]}

de Araujo, Aurigena Antunes ^{[12
]}

机构：

[1] Univ Fed Rio Grande do Norte, Dept Biophys & Pharmacol, UFRN, Postgrad Program Hlth Sci, Natal, RN, Brazil

[2] Univ Fed Rio Grande do Norte, Dept Mat Engn, UFRN, Postgrad Program Mat Engn, Natal, RN, Brazil

[3] Univ Fed Rio Grande do Norte, Dept Morphol, UFRN, Postgrad Program Hlth Sci,Postgrad Program Funct, Natal, RN, Brazil

[4] Univ Fed Rio Grande do Norte, Dept Dent, Natal, RN, Brazil

[5] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90024 USA

[6] Univ Fed Rio Grande do Norte, Dept Dent, Postgrad Program Dent Sci, Natal, RN, Brazil

[7] Percuros BV, NL-2333 CL Leiden, Netherlands

[8] Leiden Univ, Med Ctr, Translat Nanobiomat & Imaging, Dept Radiol, NL-2333 ZA Leiden, Netherlands

[9] Meikai Univ, Dept Periodontol, Saitama, Japan

[10] Univ Fed Rio Grande do Norte, Dept Biophys & Pharmacol, Postgrad Program RENORBIO, Postgrad Program Biol Sci, Natal, RN, Brazil

[11] Univ Fed Rio Grande do Norte, Dept Biophys & Pharmacol, Postgrad Program Pharmaceut Sci, Postgrad Program Biol Sci, Natal, RN, Brazil

[12] Univ Fed Rio Grande do Norte, Dept Biophys & Pharmacol, Postgrad Program Pharmaceut Sci, UCLA,Postgrad Program Dent Sci, Natal, RN, Brazil

来源：

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2020年 / 12卷 / 06期

关键词：

Zirconia; hydroxyapatite; scaffold; calvaria defect; GROWTH-FACTOR; BONE REPAIR; FRACTURE; CELLS; BIOMATERIALS; EXPRESSION; INFLAMMATION; ZIRCONIA;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The aim of this study was to characterize and evaluate zirconia/hydroxyapatite in a critical size calvarial defect model in rats. Zirconia/hydroxyapatite (80/20) scaffold was characterized by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Critical size (8 mm) calvarial defects were created in wistar rats (n=48) and divided into four groups (90 days): G0 Group: positive control; G1 Group: hydroxyapatite; G2 Group: Zirconia; G3 Group: Zirconia/hydroxyapatite (80/20). Calvaria were subjected to Micro CT, histological and immunohistochemical analyses (RANK, RANKL, OPG, osteocalcin and FGF-2). IL-1 beta, IL-10 and TNF-alpha levels were analyzed by Elisa Immunoassay. The XRD analysis confirmed the formation of a crystalline structure and SEM showed the presence of regions corresponding to Zirconia and Hydroxyapatite. The Micro CT showed increased bone volume (BV/TV) and bone mineral density (BMD) in the G3 group (P<0.05). In addition, discrete periosteal bone formation was found at the interface of the defect edge and the external surface of the scaffold in the G3 group, showing osteocytes inside and osteoblasts (P<0.05) with scarce mononuclear inflammatory cells (P<0.01) in the central region of the defect. The immunostaining was moderate for RANKL, Osteocalcin and FGF-2 in the G3 group (P<0.5), while it was intense for OPG (P<0.001). IL-1 beta levels were decreased and IL-10 levels increased (P<0.05). Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defects increased bone density, osteoblast and osteoclast cell numbers, FGF-2, osteocalcin and OPG immunostaining and IL-10 levels.

引用

页码：2439 / 2450

页数：12

共 1 条

[1] Low-level laser therapy (780nm) combined with collagen sponge scaffold promotes repair of rat cranial critical-size defects and increases TGF-, FGF-2, OPG/RANK and osteocalcin expression
de Souza de Oliveira, Lana Sarita
de Araujo, Aurigena Antunes
de Araujo Junior, Raimundo Fernandes
Galvao Barboza, Carlos Augusto
Dutra Borges, Boniek Castillo
Pereira da Silva, Jose Sandro
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 2017, 98 (02) : 75 - 85

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