Adenovirus-mediated NMDA receptor knockouts in the rat hippocampal CA1 region

被引:7
作者
Kammesheidt, A [1 ]
Kato, K [1 ]
Ito, K [1 ]
Sumikawa, K [1 ]
机构
[1] UNIV CALIF IRVINE,DEPT PSYCHOBIOL,IRVINE,CA 92697
关键词
adenovirus; antisense; in vivo; N-methyl-D-aspartate; rat;
D O I
10.1097/00001756-199702100-00011
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
ADENOVIRAL antisense constructs of the rat N-methyl-D-aspartate (NMDA) receptor subunit 1 (R1) were assessed for creating NMDAR1 knockouts in rat hippocampal CA1 regions in vivo. In situ hybridization analyses showed that virus-derived antisense transcripts were detected up to 5 weeks postinfection (p.i.). Although immunological methods failed to demonstrate a reduction of NMDA receptor protein, whole-cell recording showed that neurons in the transduced regions were deficient in NMDA receptor-mediated synaptic currents, but not alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor (AMPAR)-mediated synaptic currents. Thus, the data suggest that adenovirus technology can be used to locally knockout specific gene function for dissecting molecular mechanisms of synaptic plasticity.
引用
收藏
页码:635 / 638
页数:4
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