Scanning electrochemical microscopy for study of aptamer-thrombin interfacial interactions on gold disk microelectrodes

被引：4

作者：

Bai, Huei-Yu ^{[1
]}

Javier del Campo, F. ^{[2
]}

Tsai, Yu-Chen ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan

[2] Univ Autonoma Barcelona, Centro Nacl Microelect CNM IMB, Bellaterra 08193, Spain

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2014年 / 417卷

关键词：

Scanning electrochemical microscope; Microelectrode; Thrombin; Aptamer; ELECTRODE ARRAYS; NANOPARTICLES; SPECTROSCOPY; VOLTAMMETRY; APTASENSOR; BIOSENSOR;

D O I：

10.1016/j.jcis.2013.11.050

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A feasibility for the determination of thrombin on gold disk microelectrodes (GDMs) using scanning electrochemical microscopy (SECM) is reported. The assembly process step-by-step of thrombin aptasensor on GDMs is monitored by SECM. SECM analysis reveals the immobilization of thrombin aptamers on GDMs. The interaction between thrombin aptamers and thrombin on GDMs is imaged by SECM with feedback mode using ferrocenemethanol as an electrochemical mediator. The formation of thrombin/thrombin aptamer complex on GDMs results in a decrease in the tip peak current on spatial SECM images. This method is able to linearly and selectively detect thrombin over a linear range from 10(-12) to 10(-5) M with a detection limit of 6.07 fM. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：333 / 335

页数：3

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