Potential Pathogenicity of Aeromonas spp. Recovered in River Water, Soil, and Vegetation from a Natural Recreational Area

被引:6
作者
Guerra, Roberto M. [1 ]
Damian Maleno, Francisco [1 ]
Jose Figueras, Maria [1 ]
Pujol-Bajador, Isabel [1 ,2 ]
Fernandez-Bravo, Ana [1 ]
机构
[1] Univ Rovira & Virgili, Fac Med & Hlth Sci, Dept Basic Hlth Sci, Unit Microbiol,IISPV, Reus 43201, Spain
[2] Univ Hosp St Joan Reus, Microbiol Lab, Salut St Joan Reus Baix Camp, Reus 43204, Spain
来源
PATHOGENS | 2022年 / 11卷 / 11期
关键词
Aeromonas; river water; pathogenicity; macrophages; III SECRETION SYSTEM; ANTIBIOTIC-RESISTANCE; VIRULENCE FACTORS; LATERAL FLAGELLA; GENUS AEROMONAS; ANTIMICROBIAL SUSCEPTIBILITY; PHENOTYPIC IDENTIFICATION; ENVIRONMENTAL SOURCES; STRAINS; GENES;
D O I
10.3390/pathogens11111382
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genus Aeromonas is widely distributed in aquatic environments and is recognized as a potential human pathogen. Some Aeromonas species are able to cause a wide spectrum of diseases, mainly gastroenteritis, skin and soft-tissue infections, bacteremia, and sepsis. Currently, untreated river water is used for irrigation and recreational purposes. In this study, the Aeromonas spp. present in a river recreational environment was investigated by quantifying its presence in water, soil, and vegetation using three techniques: qPCR, plate counting in selective ADA medium, and Most Probable Number, in parallel. The presence of clones in the three types of samples was elucidated through genotyping with the ERIC-PCR technique, whereas the identification of the isolated Aeromonas was carried out by sequencing the rpoD gene. Finally, the pathogenic potential of some of the strains was explored by studying the presence and expression of virulence genes characteristic of the genus, their antimicrobial susceptibility profile, as well as the quantification of their cell damage and intracellular survival in an in vitro macrophages infection model. The results showed the presence of Aeromonas in all samples with the three quantification methods, with Aeromonas popoffii being the most prevalent species. The presence of strains with the same genotype (ERIC-PCR) was also confirmed in different samples. Some of the strains showed a high level of cell damage and intracellular bacterial survival, as well as the presence of various virulence factors. Furthermore, these strains showed resistance to some of the antibiotics tested and used therapeutically in both humans and animals. These results indicate that the presence of Aeromonas in this environment may represent a biosanitary risk that could be a public health problem.
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页数:16
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