Peroxidases Produced by New Ligninolytic Bacillus strains Isolated from Marsh and Grassland Decolourized Anthraquinone and Azo Dyes

被引：13
作者：
Falade, Ayodeji [1 ,2 ]
Mabinya, Leonard [1 ,2 ]
Okoh, Anthony [1 ,2 ]
Nwodo, Uchechukwu [1 ,2 ]
机构：
[1] Univ Ft Hare, SAMRC Microbial Water Qual Monitoring Ctr, Alice, South Africa
[2] Univ Ft Hare, Appl & Environm Microbiol Res Grp AEMREG, Dept Biochem & Microbiol, Alice, South Africa
来源：
POLISH JOURNAL OF ENVIRONMENTAL STUDIES | 2019年 / 28卷 / 05期
基金：
英国医学研究理事会; 新加坡国家研究基金会;
关键词：
bioremediation; dye decolourization; ligninolytic bacteria; lignin-modifying enzymes; BACTERIAL STRAINS; METABOLIZE LIGNIN; KRAFT-LIGNIN; DECOLORIZATION; DEGRADATION; PULP; BIODEGRADATION; PRETREATMENT;
D O I：
10.15244/pjoes/92520
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The biotechnological relevance of ligninolytic organisms remains topical and may remain so in the foreseeable future. The enzyme battery produced by ligninolytic bacteria, including Bacillus species, has shown immense industrial significance. Consequently, peroxidases produced by newly isolated ligninolytic Bacillus strains from the marsh and grassland in Hogsback forest reserve of the Eastern Cape Province of South Africa were evaluated for decolourization of anthraquinone (Remazol Brilliant Blue R-RBBR) and azo (Congo Red-CR) dyes. Maximum dye decolourization was observed with the peroxidase from Bacillus sp. NWODO-3: CR (69.89 +/- 2.64 %) and RBBR (72.12 +/- 0.38 %). Dye decolourization readings for peroxidases from the other Bacillus strains were CR: 55.06 +/- 5.48 %, RBBR: 70.45 +/- 0.0 % (Bacillus sp. MABINYA-1), 42.62 +/- 5.55 % and 42.42 +/- 4.82 % against CR for Bacillus sp. MABINYA-2 and Bacillus sp. FALADE-1, respectively. RBBR was less susceptible to the attack by crude peroxidase produced by Bacillus sp. MABINYA-2 and Bacillus sp. FALADE-1 as the dye decolourization activities observed were 4.91 +/- 0.36 % and 1.19 +/- 0.0 %, respectively. These results suggest the industrial relevance of peroxidases from the new ligninolytic Bacillus strains in bioremediation.
引用
收藏
页码：3163 / 3172
页数：10
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