Silencing of the chalcone synthase gene in Casuarina glauca highlights the important role of flavonoids during nodulation

被引:56
作者
Abdel-Lateif, Khalid [1 ]
Vaissayre, Virginie [1 ]
Gherbi, Hassen [1 ]
Verries, Clotilde [2 ]
Meudec, Emmanuelle [2 ]
Perrine-Walker, Francine [1 ]
Cheynier, Veronique [2 ]
Svistoonoff, Sergio [1 ]
Franche, Claudine [1 ]
Bogusz, Didier [1 ]
Hocher, Valerie [1 ]
机构
[1] Inst Rech Dev, UMR DIADE IRD UM2, Equipe Rhizogenese, F-34394 Montpellier 5, France
[2] INRA, Sci Oenol UMR1083, F-34060 Montpellier, France
关键词
actinorhizal symbiosis; Casuarina glauca; chalcone synthase; endosymbiosis; flavonoids; Frankia; RNA interference; signaling; SYMBIOTIC NITROGEN-FIXATION; ARBUSCULAR MYCORRHIZA FUNGI; ACTINORHIZAL NODULE; ALLOCASUARINA-VERTICILLATA; PHYLOGENETIC PERSPECTIVES; MEDICAGO-TRUNCATULA; ALNUS-GLUTINOSA; ROOT-NODULES; EXPRESSION; RHIZOBIA;
D O I
10.1111/nph.12326
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nitrogen-fixing root nodulation is confined to four plant orders, including > 14 000 Leguminosae, one nonlegume genus Parasponia and c. 200 actinorhizal species that form symbioses with rhizobia and Frankia bacterial species, respectively. Flavonoids have been identified as plant signals and developmental regulators for nodulation in legumes and have long been hypothesized to play a critical role during actinorhizal nodulation. However, direct evidence of their involvement in actinorhizal symbiosis is lacking. Here, we used RNA interference to silence chalcone synthase, which is involved in the first committed step of the flavonoid biosynthetic pathway, in the actinorhizal tropical tree Casuarina glauca. Transformed flavonoid-deficient hairy roots were generated and used to study flavonoid accumulation and further nodulation. Knockdown of chalcone synthase expression reduced the level of specific flavonoids and resulted in severely impaired nodulation. Nodule formation was rescued by supplementing the plants with naringenin, which is an upstream intermediate in flavonoid biosynthesis. Our results provide, for the first time, direct evidence of an important role for flavonoids during the early stages of actinorhizal nodulation.
引用
收藏
页码:1012 / 1021
页数:10
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