Quantitation of green fluorescent protein using a gel-based imaging method

被引：11

作者：

Chew, Few Ne ^{[1
]}

Tan, Wen Siang ^{[2
,3
]}

Ling, Tau Chuan ^{[4
]}

Tan, Chon Seng ^{[5
]}

Tey, Beng Ti ^{[1
,3
]}

机构：

[1] Univ Putra Malaysia, Fac Engn, Dept Chem & Environm Engn, Upm Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Upm Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Inst Biosci, Upm Serdang 43400, Selangor, Malaysia

[4] Univ Putra Malaysia, Fac Engn, Dept Proc & Food Engn, Upm Serdang 43400, Selangor, Malaysia

[5] Malaysia Agr Res & Dev Inst, Biotechnol Res Ctr, Serdang 43400, Selangor, Malaysia

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 384卷 / 02期

关键词：

ESCHERICHIA-COLI; MARKER;

D O I：

10.1016/j.ab.2008.10.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current Study, a gel documentation imaging system using a native polyacrylamide get for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：353 / 355

页数：3

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