Structural studies of O-specific polysaccharide(s) and biological activity toward plants of the lipopolysaccharide from Azospirillum brasilense SR8

被引:9
作者
Sigida, E. N. [1 ]
Fedonenko, Y. P. [1 ]
Shashkov, A. S. [2 ]
Toukach, P. V. [2 ]
Shelud'ko, A. V. [1 ]
Zdorovenko, E. L. [2 ]
Knirel, Y. A. [2 ]
Konnova, S. A. [1 ,3 ]
机构
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, 13 Prospect Entuziastov, Saratov 410049, Russia
[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, 47 Leninsky Prospect, Moscow 119991, Russia
[3] NG Chernyshevsky Saratov State Univ, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia
基金
俄罗斯科学基金会;
关键词
Lipopolysaccharide; O-specific polysaccharide; Structure NMR simulation; Azospirillum brasilense; Root hair deformation; Chemotaxis; WHEAT; GROWTH; CONFIGURATION; RHIZOBACTERIA; RHIZOSPHERE; INVOLVEMENT; BACTERIA;
D O I
10.1016/j.ijbiomac.2018.12.229
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipopolysaccharide (LPS) was extracted from dry bacterial cells of plant-growth-promoting bacterium Azospirillum brasilense SR8 (IBPPM 5). The O-specific polysaccharide (OPS) was obtained by mild acid hydrolysis of the lipopolysaccharide and studied by sugar analysis, H-1 and C-13 NMR spectroscopy, including H-1 H-1 COSY, TOCSY, ROESY, and H-1, C-13 HSQC and HMBC experiments, computational NMR-based structure analysis, and Smith degradation. The OPS was shown to contain two types of repeating units of the following structure: beta-D-Glcp-(1 -> 4) -> 2)-alpha-L-Rhap-(1 -> 3)-alpha-L-Rhap-(1 -> 3)-alpha-L-Rhap-(1 -> 1 beta-D-Ga1pNAc4Me-(1 ->-4) -> 4)-alpha-L-Rhap-(1 -> 3)-beta-D-ManpNAc-(1 -> 2 Both OPS structures are present in A. brasilense 54, from which structure 1 has been reported earlier (Fedonenko et al., 2011), whereas to our knowledge structure 2 has not been hitherto found in bacterial saccharides. Treatment of wheat seedling roots with LPS of A. brasilense SR8 increased the number of root hair deformations as compared to seedlings grown without LPS, but had no effect on adsorption of the bacteria to the root surface. A. brasilense SR8 was able to utilize LPS of several structurally related Azospirillum strains. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码:246 / 253
页数:8
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